Tm studies of a tertiary structure from the human hepatitis delta agent which functions in vitro as a ribozyme control element.
Tm studies of a tertiary structure from the human hepatitis delta agent which functions in vitro as a ribozyme control element.
复制标题
对人丁型肝炎剂的三级结构的研究，该结构在体外作为核酶控制元件发挥作用。
DOI：
10.1093/nar/23.21.4391
复制
发表时间：
1995
影响因子：
14.9
通讯作者：
Schreiber,DR
中科院分区：
文献类型：
--
作者：
Branch,AD;Polaskova,JA;Schreiber,DR
Viroids and other circular subviral RNA pathogens, such as the hepatitis delta agent, use a rolling circle replication cycle requiring an intact circular RNA. However, many infectious RNAs have the potential to form self-cleavage structures, whose formation must be controlled in order to preserve the circular replication template. The native structure of delta RNA contains a highly conserved element of local tertiary structure which is composed of sequences partially overlapping those needed to form the self-cleavage motif. A bimolecular complex containing the tertiary structure can be made. We show that when it is part of this bimolecular complex the potential cleavage site is protected and is not cleaved by the delta ribozyme, demonstrating that the element of local tertiary structure can function as a ribozyme control elementin vitro. Physical studies of the complex containing this element were carried out. The complex binds magnesium ions and is not readily dissociated by EDTA under the conditions tested; >50% of the complexes remain following incubation in 1 mM EDTA at 60°C for 81 min. The thermal stability of the complex is reduced in the presence of sodium ions. A DNA complex and a perfect RNA duplex studied in parallel showed a similar effect, but of lesser magnitude. The RNA complex melts at temperatures −10°C lower in buffers containing 0.5 mM MgCI2 and 100 mM NaCI than in buffers containing 0.5 mM MgCI2 with no NaCI (78.1 compared with 87.7°C). The element of local tertiary structure in delta genomic RNA appears to be a molecular clamp whose stability is highly sensitive to ion concentration in the physiological range.
