Peripheral Brain-Derived Neurotrophic Factor Modulates Regeneration Following Inferior Alveolar Nerve Injury in Rats
Peripheral Brain-Derived Neurotrophic Factor Modulates Regeneration Following Inferior Alveolar Nerve Injury in Rats
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周围脑源性神经营养因子调节大鼠下牙槽神经损伤后的再生
DOI：
10.11607/ofph.1651
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Seo K
中科院分区：
文献类型：
--
作者：
Yoshikawa H;Yamada Y;Kurose M;Yamamura K;Maeda T;Seo K
目的探讨神经损伤后局部产生的脑源性神经营养因子（BDNF）对受损神经功能再生的影响。方法切断成年雄性大鼠下牙槽神经，在损伤部位注射1 μg或10 μg BDNF抗体;第三组大鼠接受生理盐水，第四组进行神经结扎。采用实时定量聚合酶链反应（PCR）技术，对切断的组织和三叉神经节中的BDNF mRNA进行定量。术后3周测量颏区机械（触觉）刺激（使用von Frey细丝）后的头回缩阈值。结果切断后24 h内，损伤组织中BDNF mRNA的表达明显增加（非配对t检验，P<0.05），而在切断后24 h内，损伤组织中BDNF mRNA的表达明显增加（P<0.05）。01）。机械刺激引起的缩头阈值在切断后第1天先升高后降低（双向重复测量方差分析，P<0.05）。001）。术后2周，高剂量BDNF抗体组的缩头阈值高于术前（ANOVA，P<0.05）。001），但在接受较小剂量的组中没有（ANOVA，P> 0.05）。05）。生理盐水组和抗体组大鼠的JOR潜伏期和阈值均无显著性差异（非配对t检验，P> 0.05）。05).结论局部应用BDNF抗体可中和神经损伤后损伤部位的BDNF，从而影响感觉运动的恢复。J Oral Facial Pain Headache 2016; 30：346-354. doi：10.11607/ofph。1651
AimsTo examine the effects of local brain-derived neurotrophic factor (BDNF) produced after nerve injury on the functional regeneration of the damaged nerve.MethodsThe inferior alveolar nerve was transected in adult male rats and 1 µg or 10 µg of BDNF antibody was administered at the injury site; a third group of rats received saline and a fourth group underwent nerve ligation. BDNF mRNA was quantified in the transected tissue and trigeminal ganglion by using realtime polymerase chain reaction (PCR). Head withdrawal thresholds following mechanical (tactile) stimulation (with von Frey filaments) of the mental region were measured for 3 weeks postoperatively. Electromyographic activity of the jaw opening reflex (JOR) was recorded from the anterior belly of the digastric muscle.ResultsWithin 24 hours, transection induced significant elevation of BDNF mRNA expression in the injured tissue (unpaired t test, P<. 01). The head withdrawal threshold to mechanical stimulation increased at 1 day after transection and then decreased (two-way repeated measures analysis of variance [ANOVA], P<. 001). At 2 weeks after surgery, the head withdrawal threshold was higher than before surgery in the group that received a higher dose of BDNF antibody (ANOVA, P<. 001), but not in the group that received a smaller dose (ANOVA, P>. 05). No significant differences were observed in the latency or threshold of the JOR between saline-and antibody-treated rats (unpaired t test, P>. 05).ConclusionThese results suggest that locally administered BDNF antibody neutralizes nerve injury–induced BDNF at the injury site and thus influences sensorimotor recovery. J Oral Facial Pain Headache 2016; 30: 346–354. doi: 10.11607/ofph. 1651