Dynamic mechanical measurement of the viscoelasticity of single adherent cells
Dynamic mechanical measurement of the viscoelasticity of single adherent cells
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DOI：
10.1063/1.4942364
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发表时间：
2016-02-29
影响因子：
4
通讯作者：
Bashir, Rashid
中科院分区：
文献类型：
--
作者：
Corbin, Elise A.;Adeniba, Olaoluwa O.;Bashir, Rashid
最近许多关于单个细胞粘弹性的研究将力学与细胞功能和健康联系起来。在这里，我们介绍了一个测量的粘弹性的个人人类结肠癌细胞（HT-29）使用硅基座微机电系统（MEMS）谐振传感器。我们证明，可以通过测量我们的共振传感器平台的振动振幅的差异来提取单个贴壁细胞的粘弹性。使用激光多普勒振动计（LDV）测量基座传感器的振动幅度。与驱动振幅（振幅比）相比，传感器振幅的变化受到粘附细胞的机械性质的影响。固定细胞的振幅比大于活细胞，p值
Many recent studies on the viscoelasticity of individual cells link mechanics with cellular function and health. Here, we introduce a measurement of the viscoelastic properties of individual human colon cancer cells (HT-29) using silicon pedestal microelectromechanical systems (MEMS) resonant sensors. We demonstrate that the viscoelastic properties of single adherent cells can be extracted by measuring a difference in vibrational amplitude of our resonant sensor platform. The magnitude of vibration of the pedestal sensor is measured using a laser Doppler vibrometer (LDV). A change in amplitude of the sensor, compared with the driving amplitude (amplitude ratio), is influenced by the mechanical properties of the adhered cells. The amplitude ratio of the fixed cells was greater than the live cells, with a p-value