Transient immunohistochemical labelling of rat retinal axons during Wallerian degeneration by a monoclonal antibody to neurofilaments
Transient immunohistochemical labelling of rat retinal axons during Wallerian degeneration by a monoclonal antibody to neurofilaments
复制标题
神经丝单克隆抗体对华勒变性过程中大鼠视网膜轴突的瞬时免疫组织化学标记
DOI：
10.1016/0006-8993(94)91917-8
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
R. Schmidt
中科院分区：
文献类型：
--
作者：
D. Meller;U. Eysel;R. Schmidt
对照大鼠中枢视神经纤维中，高分子量神经丝蛋白上非磷酸化表位的单抗SMI32免疫组织化学标记较低。单侧视神经切断后，视束退变纤维中SMI32的标记在第2天和第4天出现短暂的强阳性表达，第8天开始下降，第21天保持较低水平。因此，SMI32免疫组织化学染色可作为大鼠视系统纤维变性的阳性标记物。
Immunohistochemical labelling with the monoclonal antibody SMI32 to non-phosphorylated epitopes on neurofilament proteins of high molecular weight class was low in rat central optic fibers of controls. After unilateral transection of optic nerve, a strong, transient increase of labelling with SMI32 occurred in degenerating fibers of optic tract at 2 and 4 days, which then declined at 8 and remained low at 21 days. Consequently, immunostaining with SMI32 may serve as a positive marker for degenerating fibers in rat optic system.