Enrichment of bacteriochlorophyll g′ in membranes of Heliobacterium chlorum by ether extraction Unequivocal evidence for its existence in vivo
Enrichment of bacteriochlorophyll g′ in membranes of Heliobacterium chlorum by ether extraction Unequivocal evidence for its existence in vivo
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通过乙醚萃取富集绿色螺杆菌膜中的细菌叶绿素 g 明确证据证明其在体内的存在
DOI：
10.1016/0014-5793(91)80778-2
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发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
J. Amesz
中科院分区：
文献类型：
--
作者：
Masami Kobayashi;Tadashi Watanabe;I. Ikegami;E. J. van de Meent;J. Amesz
H的治疗。用水饱和度较高的二乙醚制成的绿膜制剂使细菌-叶绿素(BCHL)g‘摩尔分数(通过对其丙酮提取物的高效液相色谱分析)增加到占总BChlgor的40%以上。从纯BChlg开始，BChlg的摩尔分数不应超过乙醚中平衡值的24.6%。因此，BChlg‘在体内的存在(和可能的功能)得到了明确的证明。
Treatment ofH. chlorummembrane preparations with diethyl ether of high degrees of water saturation raised the bacteriochlorophyll (BChl)g′ mole fraction, as determined by HPLC analysis of their acetone extracts, toward a level of 40% of total BChlgor higher. Starting from pure BChlg, the BChlg′ mole fraction should never exceed 24.6% which is the equilibrium value in diethyl ether. The existence (and possible functioning) of BChlg′ in vivo is thus unequivocally demonstrated.