2 HOMOLOGOUS PROTEIN-COMPONENTS OF HEPATIC GAP-JUNCTIONS
2 HOMOLOGOUS PROTEIN-COMPONENTS OF HEPATIC GAP-JUNCTIONS
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DOI：
10.1038/329732a0
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发表时间：
1987-10-22
期刊：
影响因子：
64.8
通讯作者：
REVEL, JP
中科院分区：
文献类型：
--
作者：
NICHOLSON, B;DERMIETZEL, R;REVEL, JP
间隙连接由紧密排列的跨膜通道（即连接子）组成，低相对分子质量的物质通过这些通道从细胞扩散到邻近的细胞。在肝脏中，连接子由6个蛋白顶点组成，直到现在，人们都认为它们是相同的。然而，除了相对分子质量（Mr） 28000的主要多肽（见文献4和6）外，在肝脏中反复观察到mr21000 （21K）的成分3 - 5。该蛋白的氨基末端序列（18个残基）表明它与mr28k蛋白相关，但与mr28k蛋白不同。免疫染色和免疫沉淀显示这两种蛋白位于相同的间隙连接斑块中。因此，肝间隙连接通道（延伸到其他通道）似乎是由两种（或更多）同源蛋白组成的。
Gap junctions consist of closely packed pairs of transmembrane channels, the connexons, through which materials of low relative molecular mass diffuse from the cell to neighbouring cells. In liver, connexons consist of six protein summits1,2which, until now, were believed to be identical3. However, besides the major polypeptide of relative molecular mass (Mr) 28,000 (and see refs 4 and 6), a component ofMr21,000 (21K) has been repeatedly observed3–5in liver. The amino-terminal sequence (18 residues) of this less abundant protein shows that it is related to, but distinct from, theMr28K protein. Immuno-staining and immuno-precipitation show both proteins to be in the same gap junctional plaques. Thus, it seems that hepatic gap junction channels (and by extension possibly others) are composed of two (or more) homologous proteins.