Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican.
Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican.
复制标题
从脑中克隆主要的硫酸乙酰肝素蛋白聚糖并鉴定为大鼠形式的磷脂酰肌醇蛋白聚糖。
DOI：
10.1016/0006-291x(92)92398-h
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发表时间：
1992
影响因子：
3.1
通讯作者：
Margolis,RU
中科院分区：
文献类型：
--
作者：
Karthikeyan,L;Maurel,P;Rauch,U;Margolis,RK;Margolis,RU
我们获得了高度保守的硫酸乙酰肝素蛋白多糖的完整编码序列，我们之前从大鼠脑中分离后对其进行了生化表征。 558 个氨基酸的开放阅读框编码分子量为 62 kDa 的蛋白质，包含分离的蛋白聚糖中存在的三个肽序列。获得的总序列长3.5 kb，包括1.6 kb 3'非翻译序列和0.2 kb 5'非翻译序列。推导的氨基酸序列以及 3'-和 5'-非翻译序列分别与磷脂酰肌醇锚定的人肺成纤维细胞硫酸乙酰肝素蛋白聚糖（磷脂酰肌醇蛋白聚糖）的序列具有 89% 和 66-80% 的同一性，在大量人类细胞系中可检测到其 mRNA。因此，我们的数据表明，大脑中主要的硫酸乙酰肝素蛋白多糖是大鼠形式的磷脂酰肌醇蛋白聚糖。
We have obtained the complete coding sequence of a highly conserved heparan sulfate proteoglycan which we previously characterized biochemically after isolation from rat brain. An open reading frame of 558 amino acids encodes a protein with a molecular mass of 62 kDa containing three peptide sequences present in the isolated proteoglycan. The total sequence obtained is 3.5 kb long, including 1.6 kb of 3′-untranslated sequence and 0.2 kb of 5′-untranslated sequence. The deduced amino acid sequence and the 3′- and 5′-untranslated sequences have 89% and 66–80% identity, respectively, with those of a phosphatidylinositol-anchored human lung fibroblast heparan sulfate proteoglycan (glypican) for which mRNA is detectable in a large number of human cell lines. Our data therefore demonstrate that this major heparan sulfate proteoglycan of brain is the rat form of glypican.