Configurations of microtubules in artificially activated eggs of the sea urchin Lytechinus variegatus.
Configurations of microtubules in artificially activated eggs of the sea urchin Lytechinus variegatus.
复制标题
海胆 Lytechinus variegatus 人工激活卵中微管的结构。
DOI：
10.1016/0014-4827(82)90069-6
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发表时间：
1982
影响因子：
3.7
通讯作者：
Schatten,G
中科院分区：
文献类型：
--
作者：
Bestor,TH;Schatten,G
本文用抗微管蛋白免疫荧光显微镜观察表明，卵经人工激活后，可被诱导组装胞质微管。这些微管经过三种不同的构型，然后进行失败分裂的循环。其中第一个是一个配置，其中微管被发现在一个无序的网络附近的卵皮质，逐渐增厚的微管包含层似乎是负责的向心运动的卵核，发生后不久激活。这些微管在约40分钟时被一群长的、放射状排列的微管所取代，这些微管在约70分钟时被重构以形成非极性有丝分裂器。当卵子在激活后的适当时间用微管稳定药物紫杉醇处理时，激活卵子的每种微管构型特征变得更加突出。在激活的卵子中，任何微管组织中心的权威都非常有限，因为没有看到紫菀样结构，也没有观察到微管与细胞核或卵皮质密切相关。在无钙海水中用氨激活卵（绕过皮质反应和钙瞬变的处理）诱导微管的出现，与用离子载体A23187或丁酸处理一样容易且以相同的模式，这两者都通过诱导细胞内钙释放和皮质反应来激活。
Anti-tubulin immunofluorescence microscopy is used here to demonstrate that eggs ofLytechinus variegatusare induced to assemble cytoplasmic microtubules upon artificial activation. These microtubules progress through three distinct configurations followed by cycles of abortive division. The first of these is a configuration in which microtubules are found in a disordered network near the egg cortex; the progressive thickening of the microtubule-containing layer appears to be responsible for the centripetal movement of the egg nucleus that occurs shortly after activation. These microtubules are replaced at about 40 min by a population of long, radially arrayed microtubules, which are restructured by about 70 min to form the apolar mitotic apparatus. Each of the microtubule configurations characteristic of activated eggs becomes more prominent when eggs are treated at the appropriate times after activation with the microtubule-stabilizing drug taxol. Any microtubule organizing centers within the activated egg must have very limited authority, since aster-like structures are not seen, and microtubules are not observed to be closely associated with the nucleus or egg cortex. Activation of eggs with ammonia in Ca2+-free sea water (a treatment that bypasses the cortical reaction and the Ca2+transient) induces the appearance of microtubules as readily and in the same patterns as does treatment with ionophore A23187 or butyric acid, both of which activate by inducing an intracellular calcium release and the cortical reaction.