QTL Analysis of Soybean Seed Coat Discoloration Associated with II TT Genotype
QTL Analysis of Soybean Seed Coat Discoloration Associated with II TT Genotype
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DOI：
10.2135/cropsci2010.02.0121
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发表时间：
2011-03-01
期刊：
影响因子：
2.3
通讯作者：
Takahashi, Ryoji
中科院分区：
文献类型：
--
作者：
Oyoo, Maurice E.;Benitez, Eduardo R.;Takahashi, Ryoji
大豆中的[Glyine max(L.)]，黄褐色短柔毛T基因座的显性等位基因与耐冷性有关，对耐冷性育种有一定的参考价值。具有显性I等位基因的黄种脐品种是日本人的首选品种，因为它的外观更好。然而，II TT等位基因组合使整个种皮变暗，降低了种皮的外观。本研究利用DNA分子标记对种皮变色的遗传基础进行了研究。由轻度变色大豆0518BW-8和严重变色大豆0734BW-1杂交而成的F-2群体分别于2003年和2007年在Memuro和kunneppu种植。种皮变色用二维比色仪的L*值来测量。2003年将104个简单序列重复标记分为20个1025 cM的连锁群，2007年将19个789 cM的连锁群划分为20个连锁群。利用Lg A2(8号染色体)的复合区间作图法定位了一个数量性状基因座(QTL)discol1。2003年的LOD得分为5.58，解释了22.0%的表型变异；2007年的LOD得分为5.62，解释了20.2%的变异。0518BW-8的等位基因增加了QTL上的L*值。Discol1的位置接近查尔酮合酶(CHS)多基因家族簇对应的/位点。在CHS1和CHS3基因的核苷酸序列以及CHS1和CHS3基因间隔区的序列上，两个品系之间没有序列差异。
In soybean [Glycine max (L.) Merr.], the dominant allele of the T locus for tawny pubescence is associated with chilling tolerance and is useful for breeding for chilling tolerance. Yellow hilum cultivars with the dominant I allele are preferred in Japan because of a better external appearance. However, the II TT allelic combination darkens the entire seed coat and degrades the external appearance. This study was conducted to investigate the genetic basis of seed coat discoloration using DNA markers. F-2 populations derived from crosses between soybean breeding lines 0518BW-8 with slight discoloration and 0734BW-1 with severe discoloration were grown at Memuro in 2003 and Kunneppu in 2007. Seed coat discoloration was measured by the L* value with a two-dimensional colorimeter. A total of 104 simple sequence repeat markers were classified into 20 linkage groups (LGs) spanning 1025 cM in 2003 and 19 LGs spanning 789 cM in 2007. A quantitative trait locus (QTL), discol1, was identified by composite interval mapping in LG A2 (chromosome 8) across years and locations. It had an LOD score of 5.58 explaining 22.0% of phenotypic variance in 2003 and a LOD score of 5.62 explaining 20.2% of variance in 2007. The allele from 0518BW-8 increased the L* value at the QTL. Position of discol1 was close to the / locus corresponding to the chalcone synthase (CHS) multigene family cluster. No sequence differences were observed between the two lines in nucleotide sequences of CHS1 and CHS3 genes and the intergenic region between CHS1 and CHS3.