Erythrocyte Membrane Failure by Electromechanical Stress
Erythrocyte Membrane Failure by Electromechanical Stress
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DOI：
10.3390/app8020174
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发表时间：
2018-02-01
影响因子：
2.7
通讯作者：
Liu, Jia
中科院分区：
文献类型：
--
作者：
Du, E.;Qiang, Yuhao;Liu, Jia
我们设想，通过介电电泳的生物细胞的电变形作为一种新的技术，以阐明膜失败的电和机械应力的机制的细节。在这里，我们展示了通过微流体中的叉指电极阵列在射频下通过幅度修改的电场完全控制生物细胞中的细胞单轴变形和拉伸恢复。对单独跟踪的人红细胞进行瞬态蠕变和循环实验。红细胞膜的粘弹-粘塑性变形行为和局部塑性变形的观察表明，机电应力导致不可逆的膜失效。膜失效的例子可以根据负载情况分成不同的组：机械硬化、物理损伤、从盘状细胞到棘状细胞的形态转化和全细胞溶解。这些结果表明，该技术可以潜在地用于探索受其他病理生理过程影响的红细胞膜衰竭。
We envision that electrodeformation of biological cells through dielectrophoresis as a new technique to elucidate the mechanistic details underlying membrane failure by electrical and mechanical stresses. Here we demonstrate the full control of cellular uniaxial deformation and tensile recovery in biological cells via amplitude-modified electric field at radio frequency by an interdigitated electrode array in microfluidics. Transient creep and cyclic experiments were performed on individually tracked human erythrocytes. Observations of the viscoelastic-to-viscoplastic deformation behavior and the localized plastic deformations in erythrocyte membranes suggest that electromechanical stress results in irreversible membrane failure. Examples of membrane failure can be separated into different groups according to the loading scenarios: mechanical stiffening, physical damage, morphological transformation from discocyte to echinocyte, and whole cell lysis. These results show that this technique can be potentially utilized to explore membrane failure in erythrocytes affected by other pathophysiological processes.