APOLIPOPROTEIN E5 AND E7 IN APPARENTLY HEALTHY JAPANESE MALES - FREQUENCIES AND RELATION TO PLASMA-LIPID LEVELS
APOLIPOPROTEIN E5 AND E7 IN APPARENTLY HEALTHY JAPANESE MALES - FREQUENCIES AND RELATION TO PLASMA-LIPID LEVELS
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DOI：
10.1007/bf01874050
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发表时间：
1994-09-01
期刊：
影响因子：
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通讯作者：
HAMAGUCHI, H
中科院分区：
文献类型：
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作者：
YAMANOUCHI, Y;ARINAMI, T;HAMAGUCHI, H
为了确定载脂蛋白（ape）E5和E7的频率及其与血脂水平的关系，采用双向凝胶电泳法测定了608名日本健康成年男性的载脂蛋白E表型。在2.8%的受试者中观察到载脂蛋白E5和E7，以及三种常见的猿E亚型E2、E3和E4。根据SDS/PAGE电泳迁移率将载脂蛋白E5分为两个亚型，E5 f为迁移较快型，E5 s为迁移较慢型。基因频率为：0.841; 0.095; 0.049; 0.009; 0.005编码apo E5 f的等位基因; 0.004编码apo E5 s的等位基因; 0.001。5例携带载脂蛋白E5 f的个体和11例携带载脂蛋白E7的个体为杂合子，胆固醇水平正常。此外，在任何apo E5 f或apo E7受试者中，血浆apo B和ape E水平均未升高。这些数据表明，猿E5 f和E7在日本人群中并不罕见，但在大多数杂合子中，载脂蛋白E5 f和E7均与高胆固醇血症无关。
In order to determine the frequencies of apolipoproteins (ape) E5 and E7 and their relation to plasma lipid levels, apo E phenotypes were determined in 608 healthy Japanese male adults by two-dimensional gel electrophoresis. Apo E5 and E7 were observed in 2.8% of the subjects, in addition to the three common ape E isoforms, E2, E3, and E4. Apo E5 was divided into two subtypes based on the migration rate on SDS/PAGE, E5f is the type with:h faster migration and E5s slower migration. The gene frequencies were: the epsilon 3 allele, 0.841; the epsilon 4 allele, 0.095; the epsilon 2 allele, 0.049; the epsilon 7 allele, 0.009; the epsilon 5 allele encoding apo E5f (the epsilon 5f allele), 0.004; and the epsilon 5 allele encoding ape E5s (the epsilon 5s allele), 0.001. The five individuals with apo E5f and the eleven with apo E7 were heterozygotes and normocholesterolemic. Also plasma apo B and ape E levels were not increased in any subjects with apo E5f or apo E7. The data suggests that ape E5f and E7 are not rare in the Japanese population but that neither apo E5f nor E7 are associated with hypercholesterolemia in most of the heterozygotes.