Teredinibacter turnerae gen. nov., sp nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae)
Teredinibacter turnerae gen. nov., sp nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae)
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DOI：
10.1099/ijs.0.02184-0
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发表时间：
2002-11-01
影响因子：
2.8
通讯作者：
Waterbury, J
中科院分区：
文献类型：
--
作者：
Distel, DL;Morrill, W;Waterbury, J
描述了从双壳类Teredinidae的钻木软体动物（船虫）Lyrodus pedicellatus的鳃组织中分离出的纤维素分解固氮细菌，以及从代表Teredinidae属中的9个的24个双壳类物种的鳃中分离出的具有相似特性的58个其他菌株。这些细胞是革兰氏阴性、刚性的杆状细胞 (0(.)4-0(.)6 x 3-6 mum），带有单极鞭毛。所有分离株都能够在补充有纤维素作为唯一碳和能量来源的简单矿物培养基中进行化能异养生长。木聚糖、果胶、羧甲基纤维素、纤维二糖以及各种糖和有机酸也支持生长。当培养物剧烈通气时，生长需要添加结合氮，但所有分离株都在微需氧条件下固定二氮。确定了六个分离株的最佳生长 pH、温度和盐度，分别约为 8(.)5、30-35 摄氏度和 0(.)3 M NaCl。分离物是海洋的。除了 NaCl 之外，生长还需要较高浓度的 Ca2+ 和 Mg2+，这反映了海水的化学性质。 DNA G+C 含量范围为 49 至 51 mol%。四个分离株的小亚基 rRNA 序列在 891 个位置上是相同的，属于变形菌门 γ 亚类的独特进化枝。基于形态、生理和系统发育特征以及与 Teredinid 双壳类（新属和新种 Teredinibacter Turnerae gen）的特定共生关系。十一月，SP。十一月，提出。模式菌株为T7902(T)(= ATCC 39867(T) = DSM 15152(T))。
A cellulolytic, dinitrogen-fixing bacterium isolated from the gill tissue of a wood-boring mollusc (shipworm) Lyrodus pedicellatus of the bivalve family Teredinidae and 58 additional strains with similar properties, isolated from gills of 24 bivalve species representing 9 of 14 genera of Teredinidae, are described. The cells are Gram-negative, rigid, rods (0(.)4-0(.)6 x 3-6 mum) that bear a single polar flagellum. All isolates are capable of chemoheterotrophic growth in a simple mineral medium supplemented with cellulose as a sole source of carbon and energy. Xylan, pectin, carboxymethylcellulose, cellobiose and a variety of sugars and organic acids also support growth. Growth requires addition of combined nitrogen when cultures are vigorously aerated, but all isolates fix dinitrogen under microaerobic conditions. The pH, temperature and salinity optima for growth were determined for six isolates and are approximately 8(.)5, 30-35 degreesC and 0(.)3 M NaCl respectively. The isolates are marine. In addition to NaCl, growth requires elevated concentrations of Ca2+ and Mg2+ that reflect the chemistry of seawater. The DNA G+C content ranged from 49 to 51 mol%. Four isolates were identical with respect to small-subunit rRNA sequence over 891 positions compared and fall within a unique clade in the gamma-subclass of the Proteobacteria. Based on morphological, physiological and phylogenetic characteristics and specific symbiotic association with teredinid bivalves, a new genus and species, Teredinibacter turnerae gen. nov., sp. nov., is proposed. The type strain is T7902(T)(= ATCC 39867(T) = DSM 15152(T)).