cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein.
cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein.
复制标题
DOI：
10.1016/s0006-291x(02)02132-0
复制
发表时间：
2002-09
影响因子：
3.1
通讯作者：
H. Tamada;E. Sakashita;K. Shimazaki;E. Ueno;T. Hamamoto;Y. Kagawa;H. Endo
中科院分区：
文献类型：
--
作者：
H. Tamada;E. Sakashita;K. Shimazaki;E. Ueno;T. Hamamoto;Y. Kagawa;H. Endo
神经RNA识别基序（RRM）型RNA结合蛋白在神经发育中起着重要作用。为了寻找一个新的神经RRM-type RNA结合蛋白，我们用抗RRM序列的多克隆抗体筛选大鼠脑表达文库。我们克隆并鉴定了一个属于RRM-type RNA结合蛋白家族的大鼠cDNA，命名为drb 1。在人类和小鼠中存在drb 1的同源基因。预测的氨基酸序列显示了一个476个残基的开放阅读框架，相应的分子量为53 kDa，由四个RNA结合结构域组成。drb 1基因在胎鼠（E12、E16）脑中特异性表达，并在发育过程中逐渐减少。原位杂交显示胎鼠脑内有神经元特异性信号。RNA结合实验表明，人Drb 1蛋白对poly（C）RNA具有结合偏好。这些结果表明，Drb 1是一个新的成员，神经RNA结合蛋白，表达的时空控制。
Neural RNA recognition motif (RRM)-type RNA-binding proteins play essential roles in neural development. To search for a new member of neural RRM-type RNA-binding protein, we screened rat cerebral expression library with polyclonal antibody against consensus RRM sequences. We have cloned and characterized a rat cDNA that belongs to RRM-type RNA-binding protein family, which we designate as drb1. Orthologs of drb1 exist in human and mouse. The predicted amino acid sequence reveals an open reading frame of 476 residues with a corresponding molecular mass of 53kDa and consists of four RNA-binding domains. drb1 gene is specifically expressed in fetal (E12, E16) rat brain and gradually reduced during development. In situ hybridization demonstrated neuron-specific signals in fetal rat brain. RNA-binding assay indicated that human Drb1 protein possesses binding preference on poly(C)RNA. These results indicate that Drb1 is a new member of neural RNA-binding proteins, which expresses under spatiotemporal control.