Brain transcriptome response to Streptococcus agalactiae infection and the heterogeneous regulation of neuropeptides on immune response in tilapia, Oreochromis niloticus
Brain transcriptome response to Streptococcus agalactiae infection and the heterogeneous regulation of neuropeptides on immune response in tilapia, Oreochromis niloticus
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DOI：
10.1016/j.aquaculture.2022.738222
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发表时间：
2022-04-13
期刊：
影响因子：
4.5
通讯作者：
Hou, Xitan
中科院分区：
文献类型：
--
作者：
Li, Qi;Liu, Ruonan;Hou, Xitan
脊椎动物大脑对感染的防御必须在不产生潜在有害炎症的情况下完成，因为大脑修复损伤的能力有限。神经肽是调节生理过程和行为的多功能神经元信号分子。最近，在哺乳动物中发现了它们在调节免疫反应和炎症中的潜在作用。然而，神经肽在硬骨鱼脑免疫应答介导中的作用尚不清楚。本文首次揭示了尼罗罗非鱼（Oreochromis niloticus）抗无乳链球菌（Streptococcus agalactiae）的脑转录谱。12个O.构建来自四个处理时间点（0、6、24和48小时）的尼罗脑。通过对不同时间点的脑转录组数据进行成对比较，共获得517个差异表达基因（DEG）。KEGG通路分析表明，大多数DEG处于“神经活性配体-受体相互作用”（ko 04080）。此外，还鉴定了37个神经肽家族，包含143个神经肽前体基因（产生204种不同的转录本）。15个神经肽前体基因（ADCYAP 1A、AVP、CCK 2、GAL、GNRH 3、NPY 1、OXT、PDYN、PMCH 1、PMCH 2、POMC 1A、SST 1、TAC 1、UCN 3和HAMP 1）在S.无乳感染其中，五个成熟的神经肽（AVP，OXT，UCN 3，SP和NKA）的制备和用于确定和表征免疫应答的异质性调节。具体而言，神经肽促进炎症反应、免疫途径和脂多糖（LPS）刺激的cAMP-PKA途径。然而，对脂磷壁酸（LTA）的反应首先得到促进，并很快减少神经肽治疗。本研究首次对罗非鱼神经肽进行了全面的鉴定，为深入了解神经肽在硬骨鱼类中的进化和免疫学作用奠定了基础。
Defense of the vertebrate brain against infection must be accomplished without the generation of potentially harmful inflammation since the brain has a limited capacity to repair damage. Neuropeptides are multifunctional neuronal signaling molecules that regulate physiological processes and behaviors. Recently, their potential roles in modulating immune responses and inflammation have been discovered in mammals. However, the role of neuropeptides in teleost brain immune response mediation is unknown. Herein, the brain transcriptional profiles of economically important fish, Nile tilapia (Oreochromis niloticus), against Streptococcus agalactiae, a common pathogen causing serious meningitis, was first revealed. Twelve cDNA libraries of O. niloticus brain from four treatment time points (0, 6, 24, and 48 h) were constructed. A total of 517 differentially expressed genes (DEGs) were obtained via pairwise comparison of the brain transcriptome data at different time points. KEGG pathway analysis showed that most DEGs were in "neuroactive ligand-receptor interaction" (ko04080). Besides, 37 neuropeptide families with 143 neuropeptide precursor genes (producing 204 different transcripts) were identified. Also, 15 neuropeptide precursor genes (ADCYAP1A, AVP, CCK2, GAL, GNRH3, NPY1, OXT, PDYN, PMCH1, PMCH2, POMC1A, SST1, TAC1, UCN3, and HAMP1) were differentially expressed (P < 0.05) during S. agalactiae infection. Among them, five mature neuropeptides (AVP, OXT, UCN3, SP, and NKA) were prepared and used to determine and characterize the heterogeneous regulation of immune response. Specifically, neuropeptides promoted inflammatory response, immune pathways, and the cAMP-PKA pathway to Lipopolysaccharide (LPS) stimulation. However, the responses to Lipoteichoic acid (LTA) were first promoted and soon reduced with neuropeptides treatment. This study represents the first comprehensive identification of neuropeptides in tilapia, and would contribute to a better understanding of the evolution and immunological roles of neuropeptides in teleosts.