Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding.
Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding.
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电穿孔的荧光蛋白生物传感器,用于探测无基因编码的细胞中的分子活性。
DOI:
10.1039/c4cc04730c
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发表时间:
2014-10-09
期刊:
影响因子:
--
通讯作者:
Lu C
中科院分区:
文献类型:
--
作者:
Sun C;Ouyang M;Cao Z;Ma S;Alqublan H;Sriranganathan N;Wang Y;Lu C
Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution.
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影响因子:
2.9
作者:
GRAZIADEI, L;BURFEIND, P;BARSAGI, D
通讯作者:
BARSAGI, D
影响因子:
14.8
作者:
Kardash, Elena;Bandemer, Jan;Raz, Erez
通讯作者:
Raz, Erez
影响因子:
5.3
作者:
Itoh, RE;Kurokawa, K;Matsuda, M
通讯作者:
Matsuda, M
DOI:
10.1073/pnas.0807537105
发表时间:
2008-09-23
影响因子:
11.1
作者:
Ouyang, Mingxing;Sun, Jie;Wang, Yingxiao
通讯作者:
Wang, Yingxiao
影响因子:
4.3
作者:
Lu S;Ouyang M;Seong J;Zhang J;Chien S;Wang Y
通讯作者:
Wang Y