Human B cell clones can be induced to proliferate and to switch to IgE and IgG4 synthesis by interleukin 4 and a signal provided by activated CD4+ T cell clones.
Human B cell clones can be induced to proliferate and to switch to IgE and IgG4 synthesis by interleukin 4 and a signal provided by activated CD4+ T cell clones.
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DOI：
10.1084/jem.173.3.747
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发表时间：
1991-03-01
期刊：
影响因子：
--
通讯作者：
de Vries JE
中科院分区：
文献类型：
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作者：
Gascan H;Gauchat JF;Roncarolo MG;Yssel H;Spits H;de Vries JE
本研究表明，克隆的表面IgM阳性的人B细胞在T细胞克隆和白细胞介素4 （IL-4）提供接触介导的信号后，可以被诱导增殖并以高频率切换到IgG4和IgE的产生。这种T细胞信号是非抗原特异性的，由活化的CD4+细胞提供，而活化的CD8+或静止的CD4+ T细胞克隆是无效的。用克隆的CD4+ T细胞和IL-4培养的B细胞克隆中15-35%产生抗体；35-45%产生抗体的孔中含有IgE和IgG4。除了只产生IgG4或IgE的B细胞克隆外，还观察到产生多种同型的B细胞克隆。同时产生IgG4和IgE， IgM， IgE和IgM，或IgG4和IgE，这表明在克隆扩增过程中可能发生从IgM到IgG4和IgE的连续转换。此外，克隆扩增过程中仅产生IgM、IgG4和IgE，这表明这种同型转换是由B细胞受到刺激的方式所引导的，而不是一个随机过程。
In the present study, it is demonstrated that cloned surface IgM- positive human B cells can be induced to proliferate and to switch with high frequencies to IgG4 and IgE production after a contact-mediated signal provided by T cell clones and interleukin 4 (IL-4). This T cell signal is antigen nonspecific and is provided by activated CD4+ cells, whereas activated CD8+ or resting CD4+ T cell clones are ineffective. 15-35% of the B cell clones cultured with cloned CD4+ T cells and IL-4 produced antibodies; 35-45% of those wells in which antibodies were produced contained IgE and IgG4. In addition to B cell clones that produced IgG4 or IgE only, B cell clones producing multiple isotypes were observed. Simultaneous production of IgG4 and IgE, IgM, IgE, and IgM, or IgG4 and IgE was detected, suggesting that during clonal expansion switching might occur in successive steps from IgM to IgG4 and IgE. In addition, production of only IgM, IgG4, and IgE during clonal expansion indicates that this isotype switching is directed by the way a B cell is stimulated and that it is not a stochastic process.