A role for cell sex in stem cell-mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency.
A role for cell sex in stem cell-mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency.
复制标题
DOI：
10.1083/jcb.200612094
复制
发表时间：
2007-04-09
期刊：
影响因子：
--
通讯作者：
Huard J
中科院分区：
文献类型：
--
作者：
Deasy BM;Lu A;Tebbets JC;Feduska JM;Schugar RC;Pollett JB;Sun B;Urish KL;Gharaibeh BM;Cao B;Rubin RT;Huard J
我们已经证明，将肌源性干细胞(MDSCs)移植到营养不良(MDX)小鼠体内，可以有效地再生骨骼肌。然而，MDSC群体在标记谱上表现出异质性，在再生能力上表现出多样性。我们在这里表明，细胞性别是一个很大程度上影响MDSCs再生能力的变量。我们发现雌性MDSCs(F-MDSCs)再生骨骼肌的效率更高。尽管使用了额外的分离技术和细胞克隆，但我们无法获得与雌性亚组分相似的再生能力的雄性亚组分。MDSCs再生潜力的这种差异不是直接由荷尔蒙或宿主免疫反应引起的，而可能是由于细胞应激反应的先天性别相关差异造成的。与F-MDSCs相比，男性MDSCs在暴露于过氧化氢诱导的氧化应激后分化增加，这可能导致体内供体细胞枯竭，并且F-MDSCs具有增殖优势，最终促进肌肉再生。这些发现应该会说服研究人员报告细胞性别，这在很大程度上是一个未被探索的变量，并考虑依赖一种性别的细胞的影响。
We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability in regeneration abilities. We show here that cell sex is a variable that considerably influences MDSCs' regeneration abilities. We found that the female MDSCs (F-MDSCs) regenerated skeletal muscle more efficiently. Despite using additional isolation techniques and cell cloning, we could not obtain a male subfraction with a regeneration capacity similar to that of their female counterparts. Rather than being directly hormonal or caused by host immune response, this difference in MDSCs' regeneration potential may arise from innate sex-related differences in the cells' stress responses. In comparison with F-MDSCs, male MDSCs have increased differentiation after exposure to oxidative stress induced by hydrogen peroxide, which may lead to in vivo donor cell depletion, and a proliferative advantage for F-MDSCs that eventually increases muscle regeneration. These findings should persuade researchers to report cell sex, which is a largely unexplored variable, and consider the implications of relying on cells of one sex.
登录
查看更多内容
DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
3.3
作者：
Deasy, BM;Gharaibeh, BM;Huard, J
通讯作者：
Huard, J
影响因子：
2.3
作者：
Jankowski, RJ;Huard, J
通讯作者：
Huard, J
影响因子：
56.9
作者：
JILKA, RL;HANGOC, G;MANOLAGAS, SC
通讯作者：
MANOLAGAS, SC
DOI：
10.1073/pnas.0235680100
发表时间：
2003-01-21
影响因子：
11.1
作者：
Harada, H;Pavlick, KP;Grisham, MB
通讯作者：
Grisham, MB