Repetitive myocardial stunning in pigs is associated with the increased expression of inducible and constitutive nitric oxide synthases
Repetitive myocardial stunning in pigs is associated with the increased expression of inducible and constitutive nitric oxide synthases
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DOI：
10.1016/s0008-6363(99)00149-2
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发表时间：
1999-08-15
影响因子：
10.8
通讯作者：
Hall, RJC
中科院分区：
文献类型：
--
作者：
Baker, CSR;Rimoldi, O;Hall, RJC
目的：一氧化氮（NO）对心肌功能有复杂的影响，特别是缺血再灌注后。本研究的目的是研究重复心肌昏迷对心肌NO释放和诱导型（iNOS）和组成型（eNOS） NO合成酶表达的影响。方法和结果：异丙酚麻醉的猪经历了10次2分钟的旋动脉闭塞发作（n=6）或作为假手术对照组（n=4）。脑节段缩短的测量表明，在刺激30分钟后，缺血区域的功能下降到52.5 +/- 7.3%（平均+/- s.e.m.）， 5.5小时后恢复到92+/-8.7%。假对照组的功能保持稳定。静脉-动脉[NO]在基线和再灌注6小时之间的变化在两组之间有显著差异(昏迷组为0.2+/-0.7 μ M，假手术组为-4.3+/-1.6 μ M
Objectives: Nitric oxide (NO) has complex effects on myocardial function particularly following ischaemia-reperfusion. The goal of this study was to examine the result of repetitive myocardial stunning on myocardial NO release and expression of inducible (iNOS) and constitutive (eNOS) NO synthases. Methods and results: Propofol anaesthetised pigs underwent ten, 2-min episodes of circumflex artery occlusion (n=6) or acted as sham operated controls (n=4). Measurements of segment shortening demonstrated a fall in function in the ischaemic territory to 52.5 +/- 7.3% (mean+/-S.E.M.) of baseline shortening 30 min after the stunning stimulus, recovering to 92+/-8.7% 5.5 h later. Function remained stable in sham controls. The change in venous-arterial [NO] between baseline and 6 h reperfusion was found to be significantly different between the two groups (0.2+/-0.7 in stunned vs. -4.3+/-1.6 mu M in shams; P