Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion
Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion
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DOI：
10.1007/s00595-008-3900-x
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发表时间：
2009-05-01
期刊：
影响因子：
2.5
通讯作者：
Tekin, Koray
中科院分区：
文献类型：
--
作者：
Berber, Ibrahim;Aydin, Cagatay;Tekin, Koray
为探讨水溶性抗氧化剂坦普尔对大鼠肠系膜上动脉缺血再灌注损伤的保护作用，将大鼠分为3组，每组10只。组1分离肠系膜上动脉但不结扎，组2和组3结扎肠系膜上动脉60min。之后，取出钳子，开始再灌流。第3组于再灌流开始前5min静脉注射坦普尔30 mg/kg，持续给药60min。实验2组动物的髓过氧化物酶活性、丙二醛水平、细菌易位发生率显著升高，谷胱甘肽水平显著降低。在肠系膜上动脉闭塞大鼠模型中，坦泊尔可防止细菌移位，同时消除缺血/再灌注损伤对肠组织的不良影响。
We investigated whether Tempol, a water-soluble antioxidant, prevents the harmful effects of superior mesenteric ischemia/reperfusion on intestinal tissues in rats.The rats were divided into three groups of 10. In group 1, the superior mesenteric artery (SMA) was isolated but not occluded, and in groups 2 and 3 the superior mesenteric artery was occluded for 60 min. After that, the clamp was removed and reperfusion began. In group 3, 5 min before the start of reperfusion, a bolus dose of 30 mg/kg Tempol was administered intravenously and continued at a dose of 30 mg/kg for 60 min. All animals were euthanized after 24 h and tissue samples were collected for analysis.There was a significant increase in myeloperoxidase activity, malondialdehyde levels, and the incidence of bacterial translocation in group 2, with a decrease in glutathione levels. These parameters were found to be normalized in group 3. The intestinal mucosal injury score in group 2 was significantly higher than those in groups 1 and 3.Tempol prevents bacterial translocation while precluding the harmful effects of ischemia/reperfusion injury on intestinal tissues in a rat model of superior mesenteric artery occlusion.