Transposable elements have rewired the core regulatory network of human embryonic stem cells
Transposable elements have rewired the core regulatory network of human embryonic stem cells
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DOI：
10.1038/ng.600
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发表时间：
2010-07-01
期刊：
影响因子：
30.8
通讯作者：
Bourque, Guillaume
中科院分区：
文献类型：
--
作者：
Kunarso, Galih;Chia, Na-Yu;Bourque, Guillaume
检测新的基因组控制元件对于理解整体转录调节网络至关重要。我们研究了人和小鼠胚胎干细胞中三种关键调节蛋白（POU5F1，也称为OCT4; Nanog; nanog; ctcf）的全基因组结合位置。与CTCF相反，我们发现Oct4和Nanog的结合曲线明显不同，仅与5％的区域相似。我们表明，可转座元件在人类和小鼠中贡献了多达25％的结合位点，并将新基因连接到胚胎干细胞的核心调节网络中。这些数据表明，物种特异性的转座元件已大大改变了多能干细胞的转录电路。
Detection of new genomic control elements is critical in understanding transcriptional regulatory networks in their entirety. We studied the genome-wide binding locations of three key regulatory proteins (POU5F1, also known as OCT4; NANOG; and CTCF) in human and mouse embryonic stem cells. In contrast to CTCF, we found that the binding profiles of OCT4 and NANOG are markedly different, with only similar to 5% of the regions being homologously occupied. We show that transposable elements contributed up to 25% of the bound sites in humans and mice and have wired new genes into the core regulatory network of embryonic stem cells. These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells.