Longitudinal developmental analysis of prethalamic eminence derivatives in the chick by mapping of Tbr1 in situ expression
Longitudinal developmental analysis of prethalamic eminence derivatives in the chick by mapping of Tbr1 in situ expression
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DOI：
10.1007/s00429-019-02015-3
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发表时间：
2020-01-04
影响因子：
3.1
通讯作者：
Puelles, Luis
中科院分区：
文献类型：
--
作者：
Alonso, Antonia;Maria Trujillo, Carmen;Puelles, Luis
前丘脑隆起（Prethalamiceminence，PThE）是前丘脑最背侧的亚区，相当于脊椎动物前脑发育前体模型中的前体3（p3）。在哺乳动物和鸟类胚胎中，PThE可以通过其缺乏Dlx基因表达以及通过其表达与哺乳动物相关的基因如Pax6、Tbr 2和Tbr 1而与其他前丘脑区域区分开。在小鼠胚胎中的几项研究假设PThE是迁移神经元的来源，这些神经元分布在给定的端脑中心。关于鸟类PThE，它在早期胚胎阶段作为紧凑的原基是可见的，但其形态在围产期阶段变得神秘，因此其发育过程和命运在很大程度上是未知的。在这份报告中，我们详细描述了个体发育的鸡PThE从5至15天的发展，根据形态学标准，并使用Tbr 1作为分子标记，这种结构和它的迁移细胞。我们发现，最初的PThE接触喙内侧腭，苍白杏仁核和室旁下丘脑的神经元结构域。大约从胚胎第6天开始，PThE的大小和细胞含量逐渐减少，这是由于其许多神经元衍生物向附近的下丘脑和下丘脑区域的大量切向迁移。我们的分析支持，这些迁移神经元从鸟类PThE目标端脑中心，如连合隔核，如先前所述的哺乳动物，但也斜角带和视前区，和室旁下丘脑区和下丘脑结构。
The prethalamic eminence (PThE) is the most dorsal subdomain of the prethalamus, which corresponds to prosomere 3 (p3) in the prosomeric model for vertebrate forebrain development. In mammalian and avian embryos, the PThE can be delimited from other prethalamic areas by its lack of Dlx gene expression, as well as by its expression of glutamatergic-related genes such as Pax6, Tbr2 and Tbr1. Several studies in mouse embryos postulate the PThE as a source of migratory neurons that populate given telencephalic centers. Concerning the avian PThE, it is visible at early embryonic stages as a compact primordium, but its morphology becomes cryptic at perinatal stages, so that its developmental course and fate are largely unknown. In this report, we characterize in detail the ontogeny of the chicken PThE from 5 to 15 days of development, according to morphological criteria, and using Tbr1 as a molecular marker for this structure and its migratory cells. We show that initially the PThE contacts rostrally the medial pallium, the pallial amygdala and the paraventricular hypothalamic alar domain. Approximately from embryonic day 6 onwards, the PThE becomes progressively reduced in size and cell content due to massive tangential migration of many of its neuronal derivatives towards nearby subpallial and hypothalamic regions. Our analysis supports that these migratory neurons from the avian PThE target telencephalic centers such as the commissural septal nuclei, as previously described in mammals, but also the diagonal band and preoptic areas, and hypothalamic structures in the paraventricular hypothalamic area.