A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin
A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin
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DOI：
10.1016/s1074-5521(03)00092-9
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发表时间：
2003-05-01
影响因子：
--
通讯作者：
Deng, ZX
中科院分区：
文献类型：
--
作者：
Sun, YH;Zhou, XF;Deng, ZX
用于聚醚南昌霉素生物合成的PKS基因被组织成编码两组蛋白质（分别为六个和七个ORF），但被编码差向异构酶、环氧酶和环氧化物水解酶的独立ORF分开，并且值得注意的是，独立的ACP。其中一个PIKS模块缺少相应的ACP。我们提出，氧化环化形成聚醚结构的过程发生时，聚酮链仍然锚定在独立的ACP释放前。4-O-甲基-L-玫瑰糖的生物合成及其转糖基化涉及4个基因，南昌霉素的生物合成调控既有抑制作用，也有激活作用。在读框内删除KR 6结构域产生的南昌霉素苷元与4-O-甲基-L-玫瑰糖和抗菌活性的损失，与指定的PIKS结构域催化特定的生物合成，步骤。
The PKS genes for biosynthesis of the polyether nanchangmycin are organized to encode two sets of proteins (six and seven ORFs, respectively), but are separated by independent ORFs that encode an epimerase, epoxidase, and epoxide hydrolase, and, notably, an independent ACP. One of the PIKS modules lacks a corresponding ACP. We propose that the process of oxidative cyclization to form the polyether structure occurs when the polyketide chain is still anchored on the independent ACP before release. 4-0-methyl-L-rhodinose biosynthesis and its transglycosylation involve four putative genes, and regulation of nanchangmycin biosynthesis seems to involve activation as well as repression. In-frame deletion of a KR6 domain generated the nanchangmycin aglycone with loss of 4-O-methyl-L-rhodinose and antibacterial activity, in agreement with the assignments of the PIKS domains catalyzing specific biosynthetic, steps.