Effect of DSS-induced colitis on visceral sensitivity to colorectal distension in mice
Effect of DSS-induced colitis on visceral sensitivity to colorectal distension in mice
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DOI：
10.1111/j.1365-2982.2005.00736.x
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发表时间：
2006-02-01
影响因子：
3.5
通讯作者：
Lindström, E
中科院分区：
文献类型：
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作者：
Larsson, MH;Rapp, L;Lindström, E
本研究旨在评估右旋糖酐硫酸钠 (DSS) 诱导的结肠炎对内脏敏感性的影响，内脏敏感性通过 BALB/c 和 C57Bl/6 雄性小鼠对结直肠扩张 (CRD) 的内脏运动反应 (VMR) 进行测量。通过在饮用水中添加 4% DSS 5 (C57Bl/6) 或 6-7 天 (BALB/c) 来诱导炎症。平行组用于监测组织病理学变化和内脏敏感性。在预定的日子（治疗前对照，12、16、20、30、40 和 51），使用渐增的阶段性 CRD 范式（10-60 mmHg）在有意识的小鼠中诱发伪情感内脏疼痛反应。在两种小鼠品系中，在 DSS 治疗的第 2 天和第 5 天之间出现了显着的组织病理学变化，并持续到第 12 天（P < 0.05）。第 15 天，炎症评分降低了约 50%。尽管 DSS 治疗的小鼠存在炎症证据，但在任何测试时间点，DSS 治疗的小鼠和对照组之间的 VMR 至 CRD 均未显示出差异。此外，同一组小鼠在 DSS 治疗前后没有观察到差异。总之，这些数据表明，在 BALB/c 或 C57Bl/6 雄性小鼠中，DSS 诱导的结肠炎症无论是短期还是长期都不影响内脏对 CRD 的敏感性。
The present study aimed at evaluating the effect of dextran sodium sulphate (DSS)-induced colitis on visceral sensitivity, measured as the visceromotor response (VMR) to colorectal distension (CRD) in BALB/c and C57Bl/6 male mice. Inflammation was induced by the addition of 4% DSS to the drinking water for 5 (C57Bl/6) or 6-7 days (BALB/c). Parallel groups were used to monitor histopathological changes and visceral sensitivity. Pseudo-affective visceral pain responses were evoked using an increasing phasic CRD paradigm (10-60 mmHg) in conscious mice on predetermined days (pretreatment controls, 12, 16, 20, 30, 40 and 51). In both mouse strains, significant histopathological changes developed between days 2 and 5 of DSS treatment, and persisted until day 12 (P < 0.05). On day 15, inflammatory scores were reduced by about 50%. Despite evidence of inflammation in DSS-treated mice, no differences could be shown in the VMR to CRD between DSS-treated mice and controls at any time point tested. In addition, no differences were seen before and after DSS treatment in the same group of mice. In conclusion, these data suggest that DSS-induced colonic inflammation does not affect the visceral sensitivity to CRD, neither at short or long term, in BALB/c or C57Bl/6 male mice.