The MTase15 regulates reproduction in the wing-dimorphic planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
The MTase15 regulates reproduction in the wing-dimorphic planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
复制标题
MTase15 调节翅二型飞虱 Nilaparvata lugens（半翅目：飞虱科）的繁殖
DOI：
10.1111/imb.12591
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发表时间：
2019
影响因子：
2.6
通讯作者：
Xu H-J
中科院分区：
文献类型：
--
作者：
Xu N.;Chen H-H;Xue W-H;Yuan X-B;Xia P-Z;Xu H-J
S‐Adenosyl‐l‐methionine‐dependent methyltransferases (SAMMTases) modulate important cellular and metabolic activities in both prokaryotes and eukaryotes. Here, we functionally characterized an SAMMTase gene (MTase15) in the migratory brown planthopper (BPH),Nilaparvata lugens, which is the most notorious rice pest in Asia. The cDNA sequence ofMTase15is 2764 nt in length with an open reading frame of 1218 nt encoding 405 amino acid residues. Quantitative real‐time PCR analysis showed thatMTase15was readily detected from egg to adult stages and extensively distributed in various body parts of adult females and males, with slightly high levels in ovary and testis, respectively. In addition,MTase15was transcriptionally regulated by the insulin signalling pathway in BPH. RNA‐interference‐mediated knockdown ofMTase15(dsMtase15) resulted in deficiencies in vitellogenin synthesis and oogenesis, and female infertility. Males withMtase15knockdown retained the capability of producing sperms with normal viability, but less sperm was transferred to wild‐type (wt) females during copulation, and eggs laid by thesewtfemales arrested embryogenesis. These findings not only assign a functional role toMTase15, but also provide a link between the insulin signalling pathway and epigenetic regulation in BPH reproduction.
