Regional distribution of metallothionein, zinc, and copper in the brain of different strains of rats
Regional distribution of metallothionein, zinc, and copper in the brain of different strains of rats
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DOI：
10.1007/bf02783866
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发表时间：
1999-08-01
影响因子：
3.9
通讯作者：
Cherian, MG
中科院分区：
文献类型：
--
作者：
Ono, SI;Cherian, MG
确定了大脑中九个解剖区域（嗅球、皮质、纹状体、海马、丘脑加下丘脑、笔加延髓、小脑、中脑和白质）金属硫蛋白（MT）、锌和铜的区域分布，并在两种不同品系的大鼠（Sprague-Dawley [SD]和Lewis）之间进行了比较。两种品系之间的全脑MT水平没有观察到显着差异（SD大鼠为17.8+/-3.4μg/g，Lewis大鼠为20.3+/-2.3μg/g）。然而，在 SD 大鼠中，MT 在白质中的表达水平高于其他研究区域。相比之下，Lewis 大鼠的皮质中 MT 浓度最高，嗅球中最低。 SD大鼠皮质、纹状体、海马、丘脑和下丘脑的MT水平显着低于Lewis大鼠。在这两种品系中，嗅球中的锌和铜含量均明显高于其他区域（SD大鼠中的锌为27.9+/-6.8μg/g，Lewis大鼠中的锌为27.6+/-6.9μg/g，SD大鼠中的铜为5.2+/-1.5μg/g，Lewis大鼠中的铜为11.1+/-4.8μg/g）。在 SD 和 Lewis 大鼠中，第二高的锌水平出现在海马体中，而第二高的铜水平则出现在纹状体中。嗅球中锌和铜的高含量并不伴随着高MT浓度。这些结果表明，在 MT 和金属分布研究中应考虑大鼠的应变以及解剖脑区域。然而，SD 和 Lewis 大鼠的嗅球中锌和铜的最高浓度是相同的。 MT 和嗅球中金属水平之间的差异表明，除了 MT 之外，其他蛋白质在锌和铜的稳态控制中也发挥着作用。
The regional brain distribution of metallothionein (MT), zinc, and copper in the brain was determined in nine anatomical regions (olfactory bulb, cortex, corpus striatum, hippocampus, thalamus plus hypothalamus, pens plus medulla oblongata, cerebellum, midbrain, and white matter) and was compared between two different strains of rat (Sprague-Dawley [SD] and Lewis). No significant difference was observed in the whole-brain MT level between the two strains (17.8 +/- 3.4 mu g/g in SD rats and 20.3 +/- 2.3 mu g/g in Lewis rats). In SD rats, however, MT was more highly expressed in the white matter than in the other regions studied. In contrast, MT concentration was highest in the cortex and lowest in the olfactory bulb in Lewis rats. The MT levels in the cortex, corpus striatum, hippocampus, and thalamus plus hypothalamus were significantly lower in SD rats than in Lewis rats. In both strains, the olfactory bulb contained markedly higher levels of both zinc and copper than the other regions (27.9 +/- 6.8 mu g/g zinc in SD rats and 27.6 +/- 6.9 mu g/g zinc in Lewis rats, and 5.2 +/- 1.5 mu g/g copper in SD rats and 11.1 +/- 4.8 mu g/g copper in Lewis rats). The next high-est zinc levels were seen in the hippocampus, whereas the next highest copper levels were in the corpus striatum in both SD and Lewis rats. The high levels of zinc and copper in the olfactory bulb were not accompanied by concomitant high MT concentrations. These results indicate that the strain of rat as well as the anatomical brain region should be taken into account in MT and metal distribution studies. However, the highest concentrations of zinc and copper in olfactory bulb were common to both SD and Lewis rats. The discrepancy between MT and the metal levels in olfactory bulb suggests a role for other proteins in addition to MT in the homeostatic control of zinc and copper.