Accessing Raman spectral variability in human stratum corneum for quantitative in vitro depth profiling
Accessing Raman spectral variability in human stratum corneum for quantitative in vitro depth profiling
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DOI：
10.1002/jrs.4428
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Windbergs, Maike
中科院分区：
文献类型：
--
作者：
Franzen, Lutz;Windbergs, Maike
利用共聚焦拉曼显微镜对人体皮肤内的物质进行无标记和非破坏性检测的机会代表了研究药物渗透和渗透的新方法。几项研究已经介绍了共焦拉曼显微镜深度剖析皮肤，然而，报告的结果显示高偏差。因此，分析人体皮肤本身的光谱变异性是通过共聚焦拉曼显微镜对药物渗透和渗透进行系统定量研究的必要前提。在我们的工作中，我们获得了拉曼深度轮廓从切除的人体皮肤样本后，腹部整形手术和调查的绝对强度波动的四个主要的皮肤来源的拉曼峰。结果证明了光谱强度的预期高可变性，但我们无法检测到不同皮肤供体之间的差异。主要内源性皮肤成分的详细分析揭示了空间分布的差异，从而影响其作为相对深度分析的参考峰的个体适用性。此外，我们发现在更深的角质层中信号可变性增加，这在未来的物质深度分析研究中必须考虑。此外，我们发现了所有主要皮肤成分的拉曼信号的指数衰减，说明了生物组织内的信号衰减。基于这种数学描述，可以实现对人体皮肤中物质的定量跟踪。总而言之，本研究的结果阐明了充分了解人体皮肤内的内源性光谱特性的必要性，这是合理分析人体皮肤中物质深度的必要前提。版权所有（c）2013约翰威利父子有限公司
The opportunity of label-free and non-destructive detection of substances inside human skin by confocal Raman microscopy represents a novel approach for investigating drug penetration and permeation. Several studies already introduced confocal Raman microscopy for depth profiling in skin; however, the reported results show high deviations. Thus, analysis of the spectral variability of human skin itself is a necessary prerequisite for systematic quantitative investigations of drug penetration and permeation by confocal Raman microscopy. In our work, we acquired Raman depth profiles from excised human skin samples after abdominal plastic surgery and investigated the absolute intensity fluctuation of four major skin derived Raman peaks. The results prove the expected high variability in spectral intensity, but we could not detect dissimilarities between different skin donors. A detailed analysis of the major endogenous skin components revealed differences in spatial distribution which consequently affects their individual applicability as reference peaks for relative depth profiling. Furthermore, we discovered an increase in signal variability in deeper stratum corneum layers, which has to be considered in future substance depth profiling investigations. In addition, we discovered an exponential decay of the Raman signal for all major skin components accounting for signal attenuation inside biological tissue. Based on this mathematical description, quantitative follow-up of substances in human skin can be realized. All in all, the results of this study elucidate the necessity of substantial understanding of endogenous spectral characteristics inside human skin as essential prerequisite for rational depth profiling of substances in human skin. Copyright (c) 2013 John Wiley & Sons, Ltd.