Time series metagenomic sampling of the Thermopyles, Greece, geothermal springs reveals stable microbial communities dominated by novel sulfur‐oxidizing chemoautotrophs
Time series metagenomic sampling of the Thermopyles, Greece, geothermal springs reveals stable microbial communities dominated by novel sulfur‐oxidizing chemoautotrophs
复制标题
对希腊温泉关地热泉的时间序列宏基因组采样揭示了以新型硫氧化化学自养生物为主的稳定微生物群落
DOI：
10.1111/1462-2920.15373
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发表时间：
2020
影响因子：
5.1
通讯作者：
Kormas, K. A.
中科院分区：
文献类型：
--
作者：
Meziti, A.;Nikouli, E.;Hatt, J. K.;Konstantinidis, K. T.;Kormas, K. A.
地热温泉基本上不受地面环境条件的影响，因为它们是由地下水持续供应的，化学成分几乎没有变化。因此，它们的微生物群落组成和功能的变化，特别是在很长一段时间内，预计是有限的，但这一假设尚未得到严格的验证。为了缩小这一知识差距，我们对2010年至2016年从希腊中部Thermopyles富硫地热泉收集的17个水样进行了全宏基因组测序。宏基因组中16S rRNA基因片段显示，Epsilonproteobacteria相关的操作分类单位（operational taxonomic units， OTU）在大多数样品中占主导地位，基于OTU丰度的样品分组没有明显的季节性规律。样品之间在功能基因含量上的相似性很高，所有样品在功能途径上的相似性都达到了70%左右。宏基因组组装基因组（MAGs）分析进一步证实了这些群落范围内的模式，这表明在春季中，化学自养弯曲菌目的新种和属占主导地位。这些mag携带不同的硫代硫酸盐或硫化物氧化途径与碳固定途径耦合。总体而言，我们的研究表明，即使从长期来看，在中等高温的陆地春季，微生物群落的功能保持稳定，可能会推动相应的群落结构稳定。
Geothermal springs are essentially unaffected by environmental conditions aboveground as they are continuously supplied with subsurface water with little variability in chemistry. Therefore, changes in their microbial community composition and function, especially over a long period, are expected to be limited but this assumption has not yet been rigorously tested. Toward closing this knowledge gap, we applied whole metagenome sequencing to 17 water samples collected between 2010 and 2016 from the Thermopyles sulfur‐rich geothermal springs in central Greece. As revealed by 16S rRNA gene fragments recovered in the metagenomes,Epsilonproteobacteria‐related operational taxonomic units (OTUs) dominated most samples and grouping of samples based on OTU abundances exhibited no apparent seasonal pattern. Similarities between samples regarding functional gene content were high, with all samples sharing >70% similarity in functional pathways. These community‐wide patterns were further confirmed by analysis of metagenome‐assembled genomes (MAGs), which showed that novel species and genera of the chemoautotrophicCampylobacteralesorder dominated the springs. These MAGs carried different pathways for thiosulfate or sulfide oxidation coupled to carbon fixation pathways. Overall, our study showed that even in the long term, functions of microbial communities in a moderately hot terrestrial spring remain stable, presumably driving the corresponding stability in community structure.