Evidence for oligodendrocyte progenitor cell heterogeneity in the adult mouse brain.
Evidence for oligodendrocyte progenitor cell heterogeneity in the adult mouse brain.
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成年小鼠脑中少突胶质细胞祖细胞异质性的证据。
DOI：
10.1038/s41598-022-17081-7
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发表时间：
2022-07-28
影响因子：
4.6
通讯作者：
Gaultier, Alban
中科院分区：
文献类型：
--
作者：
Beiter, Rebecca M.;Rivet-Noor, Courtney;Merchak, Andrea R.;Bai, Robin;Johanson, David M.;Slogar, Erica;Sol-Church, Katia;Overall, Christopher C.;Gaultier, Alban
少突胶质细胞祖细胞（OPCs）约占成人大脑的5%，并且历史上已经研究了它们在髓鞘形成中的作用。在成人脑中，OPCs在整个成年期保持其增殖能力和分化成少突胶质细胞的能力，即使相对较少的成熟少突胶质细胞在发育后髓鞘形成中产生。最近的工作已经开始证明，OPCs可能在体内平衡和疾病中发挥多种功能，并且可以显着影响行为表型，如食物摄入和抑郁症状。然而，OPCs影响大脑功能的确切机制仍不清楚。进一步探索OPC功能的第一步是分析转录库并评估成人OPC的异质性。在这项工作中，我们证明，成人OPCs是转录多样性和分离成两个不同的群体在稳态的大脑。这两组显示出不同的转录特征和丰富的生物过程独特的个人OPC人口。我们已经验证了这些OPC人群使用多种方法，包括多重RNA原位杂交和RNA流式细胞术。这项研究提供了一个重要的资源，配置文件成人OPC的转录组，并将提供一个工具箱，为进一步研究新的OPC功能。
Oligodendrocyte progenitor cells (OPCs) account for approximately 5% of the adult brain and have been historically studied for their role in myelination. In the adult brain, OPCs maintain their proliferative capacity and ability to differentiate into oligodendrocytes throughout adulthood, even though relatively few mature oligodendrocytes are produced post-developmental myelination. Recent work has begun to demonstrate that OPCs likely perform multiple functions in both homeostasis and disease and can significantly impact behavioral phenotypes such as food intake and depressive symptoms. However, the exact mechanisms through which OPCs might influence brain function remain unclear. The first step in further exploration of OPC function is to profile the transcriptional repertoire and assess the heterogeneity of adult OPCs. In this work, we demonstrate that adult OPCs are transcriptionally diverse and separate into two distinct populations in the homeostatic brain. These two groups show distinct transcriptional signatures and enrichment of biological processes unique to individual OPC populations. We have validated these OPC populations using multiple methods, including multiplex RNA in situ hybridization and RNA flow cytometry. This study provides an important resource that profiles the transcriptome of adult OPCs and will provide a toolbox for further investigation into novel OPC functions.
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影响因子：
9
作者：
Bonfanti E;Gelosa P;Fumagalli M;Dimou L;Viganò F;Tremoli E;Cimino M;Sironi L;Abbracchio MP
通讯作者：
Abbracchio MP
影响因子：
64.8
作者：
Bergles, DE;Roberts, JDB;Jahr, CE
通讯作者：
Jahr, CE
影响因子：
32.4
作者：
Hammond, Timothy R.;Dufort, Connor;Stevens, Beth
通讯作者：
Stevens, Beth
影响因子：
11.8
作者：
DeSisto J;O'Rourke R;Jones HE;Pawlikowski B;Malek AD;Bonney S;Guimiot F;Jones KL;Siegenthaler JA
通讯作者：
Siegenthaler JA
影响因子：
25
作者：
Chen, Ying;Wu, Heng;Wang, Shuzong;Koito, Hisami;Li, Jianrong;Ye, Feng;Hoang, Jenny;Escobar, Sabine S.;Gow, Alexander;Arnett, Heather A.;Trapp, Bruce D.;Karandikar, Nitin J.;Hsieh, Jenny;Lu, Q. Richard
通讯作者：
Lu, Q. Richard