Proteome Dynamics of the Specialist Oxalate Degrader Oxalobacter formigenes.
Proteome Dynamics of the Specialist Oxalate Degrader Oxalobacter formigenes.
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DOI：
10.4172/jpb.1000384
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Knight J
中科院分区：
文献类型：
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作者：
Ellis ME;Mobley JA;Holmes RP;Knight J
产草酸杆菌是一种独特的肠道生物，它依靠草酸盐降解来满足其大部分能量和碳需求。缺乏定植是草酸钙肾结石病的危险因素。 O. formigenes 基因组序列的发布为加深我们对 O. formigenes 生物学的了解提供了机会。本研究使用基于质谱的鸟枪蛋白质组学来检查与生长从对数期到稳定期过渡相关的蛋白质水平的变化。在 O. formigenes 菌株 OxCC13 基因组中的 1867 个独特蛋白质编码基因中，检测到了 1822 个蛋白质，这位于自由生活细菌中发现的 1500-7500 个蛋白质范围的较低端。从这里提供的蛋白质数据集可以清楚地看出，O. formigenes 包含肠道微生物预期的一系列代谢途径，使其能够生存并适应新环境。尽管需要进一步的实验测试来确认介导营养变化适应的生理和调节过程，但此处提供的 O. formigenes 蛋白质数据集可用作研究不同条件下蛋白质组动力学的参考，并且具有发展假设的巨大潜力。
Oxalobacter formigenes is a unique intestinal organism that relies on oxalate degradation to meet most of its energy and carbon needs. A lack of colonization is a risk factor for calcium oxalate kidney stone disease. The release of the genome sequence of O. formigenes has provided an opportunity to increase our understanding of the biology of O. formigenes. This study used mass spectrometry based shotgun proteomics to examine changes in protein levels associated with the transition of growth from log to stationary phase. Of the 1867 unique protein coding genes in the genome of O. formigenes strain OxCC13, 1822 proteins were detected, which is at the lower end of the range of 1500–7500 proteins found in free-living bacteria. From the protein datasets presented here it is clear that O. formigenes contains a repertoire of metabolic pathways expected of an intestinal microbe that permit it to survive and adapt to new environments. Although further experimental testing is needed to confirm the physiological and regulatory processes that mediate adaptation with nutrient shifts, the O. formigenes protein datasets presented here can be used as a reference for studying proteome dynamics under different conditions and have significant potential for hypothesis development.