Fluorouracil therapy for proliferative vitreoretinopathy after vitrectomy.
Fluorouracil therapy for proliferative vitreoretinopathy after vitrectomy.
复制标题
氟尿嘧啶治疗玻璃体切除术后增殖性玻璃体视网膜病变。
DOI：
10.1016/0002-9394(83)90452-x
复制
发表时间：
1983
影响因子：
4.2
通讯作者：
O'Donnell,JJ
中科院分区：
文献类型：
--
作者：
Stern,WH;Lewis,GP;Erickson,PA;Guerin,CJ;Anderson,DH;Fisher,SK;O'Donnell,JJ
氟尿嘧啶有效抑制玻璃体切除术后视网膜前膜形成和牵引性视网膜脱离。当每 24 小时眼内注射 0.5 mg 氟尿嘧啶，持续 7 天时，在眼内注射 200,000 个培养的视网膜色素上皮细胞两周后，12 只对照眼中有 12 只眼发生牵拉性视网膜脱离，但用氟尿嘧啶治疗的 14 只眼中只有 6 只发生 (P<.001)。细胞注射 4 周后，用氟尿嘧啶治疗的 12 只眼中的 8 只出现牵拉性视网膜脱离，而对照组的 12 只眼中则有 12 只出现牵拉性视网膜脱离 (P<.001)。眼内注射200,000个培养的视网膜色素上皮细胞后4周，在连续7天每24小时使用0.5 mg氟尿嘧啶治疗的眼睛中，与对照眼相比，牵引性视网膜脱离的高度减少了50% (P<.001)。当注射的视网膜色素上皮细胞的数量增加到400,000个细胞并眼内给予1.25 mg氟尿嘧啶时，牵引性视网膜脱离的高度减少了50%每 24 小时一次，连续 7 天，注射后两周，对照组 15 只眼睛中的 15 只发生牵引性视网膜脱离，但治疗组 10 只眼睛中没有发生牵引性视网膜脱离。细胞注射后 4 周，对照组 8 只眼中的 8 只和氟尿嘧啶治疗组 5 只眼中的 5 只出现脱离，且两组脱离的平均高度相等。注射 200,000 个培养的视网膜色素上皮细胞并用氚化胸苷标记 2 小时的眼睛的视网膜前膜放射自显影显示，细胞注射两周后，0.8% 的视网膜前细胞核被标记，但经氟尿嘧啶处理的眼睛中不存在标记细胞。在细胞注射后三周，首次注意到经氟尿嘧啶处理的眼睛中视网膜前细胞的氚胸苷标记。氟尿嘧啶治疗眼中氚化胸苷标记的存在与视网膜前细胞数量的增加和牵引性视网膜脱离发生率的增加相关。
Fluorouracil effectively inhibits epiretinal membrane formation and traction retinal detachment after vitrectomy surgery. When 0.5 mg of fluorouracil was administered intraocularly every 24 hours for seven days, traction retinal detachment two weeks after the intraocular injection of 200,000 cultured retinal pigment epithelial cells occurred in 12 of 12 control eyes but in only six of 14 eyes treated with fluorouracil (P<.001). Four weeks after cell injection, eight of 12 eyes treated with fluorouracil had traction retinal detachments whereas 12 of 12 control eyes did (P<.001). The height of the traction retinal detachment four weeks after intraocular injection of 200,000 cultured retinal pigment epithelial cells was reduced 50% in eyes treated with 0.5 mg of fluorouracil every 24 hours for seven days compared to control eyes (P<.001).When the number of injected retinal pigment epithelial cells was increased to 400,000 cells and 1.25 mg of fluorouracil was administered intraocularly every 24 hours for seven days, traction retinal detachment two weeks after injection occurred in 15 of 15 eyes in the control group but in none of ten eyes in the treated group. Four weeks after cell injection, eight of eight eyes in the control group and five of five eyes in the fluorouracil-treated group had detachments and the mean height of the detachments in the two groups was equal.Autoradiography of the epiretinal membranes in eyes injected with 200,000 cultured retinal pigment epithelial cells and labeled for two hours with tritiated thymidine showed that 0.8% of the epiretinal cell nuclei were labeled two weeks after cell injection but that no labeled cells were present in the fluorouracil-treated eyes. Tritiated thymidine labeling of epiretinal cells in the fluorouracil-treated eyes was first noted three weeks after the cell injection. The presence of tritiated thymidine labeling in the fluorouracil-treated eyes correlated with an increase in the number of epiretinal cells and an increase in the incidence of traction retinal detachment.