Culture-independent analysis of desulfovibrios in the human distal colon of healthy, colorectal cancer and polypectomized individuals
Culture-independent analysis of desulfovibrios in the human distal colon of healthy, colorectal cancer and polypectomized individuals
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DOI：
10.1111/j.1574-6941.2009.00709.x
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发表时间：
2009-08-01
影响因子：
4.2
通讯作者：
Marchesi, Julian R.
中科院分区：
文献类型：
--
作者：
Scanlan, Pauline D.;Shanahan, Fergus;Marchesi, Julian R.
硫酸盐还原菌（SRB）代谢的最终产物硫化氢的产生被认为是胃肠道疾病的潜在病原体。进行定量PCR（Q-PCR）测定以从两个胃肠道疾病组（结肠直肠癌（CRC）n=27和息肉切除个体（PP）n=27）和两个健康对照组（老年人（H1）n=8和年轻人（H2）n=30）中计数噬菌体。利用亚硫酸盐还原酶（dsrAB）基因作为分子标记对脱硫弧菌的多样性进行了分析。Q-PCR在所有样品中检测到脱硫弧菌，PP、H1、H2没有观察到显著差异，脱硫弧菌的基因拷贝数平均为10（6）g（-1）粪便。与PP和H2组相比，CRC组（10（5）g（-1））的脱硫弧菌数量显著减少（P < 0.05）。多样性分析表明，脱硫弧菌物种多样性低和优势的脱硫弧菌猪是健康和疾病组的一个特点。此外，还回收了与革兰氏阳性SRB远亲的dsrAB基因序列，突出了培养独立技术对于进一步了解人类胃肠道生态系统多样性的重要性。
The production of hydrogen sulphide, an end product of metabolism by the sulphate-reducing bacteria (SRB) has been cited as a potential aetiological agent in gastrointestinal disease. Quantitative PCR (Q-PCR) assays to enumerate desulfovibrios from two gastrointestinal disease groups: colorectal cancer (CRC) n=27 and polypectomized individuals (PP) n=27, and two healthy control groups, elderly (H1) n=8 and young adults (H2) n=30 was performed. Analysis of Desulfovibrio sp. diversity using the dissimilarity sulphite reductase (dsrAB) gene as a molecular marker was also undertaken. Q-PCR detected Desulfovibrio sp. in all samples and no significant difference was observed for PP, H1, H2 with gene copy numbers of Desulfovibrio sp. averaging at 10(6) g(-1) of faeces. Significantly reduced numbers of Desulfovibrio sp. were observed for CRC (10(5) g(-1)) compared with both PP and H2 groups (P < 0.05). Diversity analysis indicated that a low Desulfovibrio sp. diversity and the predominance of Desulfovibrio piger was a feature of both healthy and disease groups. In addition, a dsrAB gene sequence distantly related to a Gram-positive SRB was also recovered, highlighting the importance of cultivation-independent techniques for furthering our understanding of the diversity of the human gastrointestinal ecosystem.