Characterisation of Walker 256 breast carcinoma cells from two tumour cell banks as assessed using two models of secondary brain tumours
Characterisation of Walker 256 breast carcinoma cells from two tumour cell banks as assessed using two models of secondary brain tumours
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DOI：
10.1186/1475-2867-13-5
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发表时间：
2013-02-01
影响因子：
5.8
通讯作者：
Ghabriel, Mounir N.
中科院分区：
文献类型：
--
作者：
Lewis, Kate M.;Harford-Wright, Elizabeth;Ghabriel, Mounir N.
背景：转移性脑瘤是乳腺癌进展的常见终末期，具有显著的相关发病率和高死亡率。Walker 256是一种与Wistar大鼠同基因的大鼠乳腺癌细胞系，常用于诱发继发性脑肿瘤。尽管最近的研究表明，来自不同细胞库的同一肿瘤细胞株在体外可能会随着时间的推移而改变其特性。方法：本研究采用颈内动脉注射和继发性脑肿瘤直接脑接种模型来确定来自美国类型培养库(ATCC)和东北大学医学研究中心(CRCTU)两个细胞库的Walker 256细胞的致瘤性。结果：肿瘤的发生率和体积以及白蛋白、IBA1和GFAP的免疫阳性反应被用作肿瘤成瘤性和肿瘤与宿主脑微环境相互作用的指标。与ATCC细胞系相比，CRCTU Walker 256细胞具有更高的发病率、更大的肿瘤体积、显著的血脑屏障破坏和显著的胶质反应。结论：从不同细胞库获得的永生化癌细胞株在体内可能具有不同的特征和行为。
Background: Metastatic brain tumours are a common end stage of breast cancer progression, with significant associated morbidity and high mortality. Walker 256 is a rat breast carcinoma cell line syngeneic to Wistar rats and commonly used to induce secondary brain tumours. Previously there has been the assumption that the same cancer cell line from different cell banks behave in a similar manner, although recent studies have suggested that cell lines may change their characteristics over time in vitro.Methods: In this study internal carotid artery injection and direct cerebral inoculation models of secondary brain tumours were used to determine the tumorigenicity of Walker 256 cells obtained from two cell banks, the American Type Culture Collection (ATCC), and the Cell Resource Centre for Medical Research at Tohoku University (CRCTU).Results: Tumour incidence and volume, plus immunoreactivity to albumin, IBA1 and GFAP, were used as indicators of tumorigenicity and tumour interaction with the host brain microenvironment. CRCTU Walker 256 cells showed greater incidence, larger tumour volume, pronounced blood-brain barrier disruption and prominent glial response when compared to ATCC cell line.Conclusions: These findings indicate that immortalised cancer cell lines obtained from different cell banks may have diverse characteristics and behaviour in vivo.