Induction of metaphase arrest in cleaving Xenopus embryos in by the protein kinase p90Rsk
Induction of metaphase arrest in cleaving Xenopus embryos in by the protein kinase p90Rsk
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DOI：
10.1126/science.286.5443.1365
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发表时间：
1999-11-12
期刊：
影响因子：
56.9
通讯作者：
Maller, JL
中科院分区：
文献类型：
--
作者：
Gross, SD;Schwab, MS;Maller, JL
在受精前，脊椎动物卵被细胞生长抑制因子（CSF）阻滞在减数分裂II的中期，CSF是一种需要激活丝裂原活化蛋白激酶（MAPK）途径的活性。为了研究CSF阻滞是否由蛋白激酶p90（Rsk）介导，该蛋白激酶p90被MAPK磷酸化并激活，在爪蟾胚胎中表达了组成型激活（CA）形式的Rsk。CA Rsk的表达导致卵裂停滞，细胞学分析表明，停滞的卵裂球处于M期，具有明显的减数分裂中期纺锤体特征。因此，Rsk似乎是介导的MAPK依赖性CSF逮捕脊椎动物未受精卵。
Before fertilization, vertebrate eggs are arrested in metaphase of meiosis II by cytostatic factor (CSF), an activity that requires activation of the mitogen-activated protein kinase (MAPK) pathway. To investigate whether CSF arrest is mediated by the protein kinase p90(Rsk), which is phosphorylated and activated by MAPK, a constitutively activated (CA) form of Rsk was expressed in Xenopus embryos. Expression of CA Rsk resulted in cleavage arrest, and cytological analysis showed that arrested blastomeres were in M phase with prominent spindles characteristic of meiotic metaphase. Thus, Rsk appears to be the mediator of MAPK-dependent CSF arrest in vertebrate unfertilized eggs.