Ciboulot regulates actin assembly during Drosophila brain metamorphosis
Ciboulot regulates actin assembly during Drosophila brain metamorphosis
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DOI：
10.1016/s0092-8674(00)00068-4
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发表时间：
2000-09-15
期刊：
影响因子：
64.5
通讯作者：
Préat, T
中科院分区：
文献类型：
--
作者：
Boquet, I;Boujemaa, R;Préat, T
动态的肌动蛋白细胞骨架对于发育过程中细胞形状的重塑至关重要，但许多肌动蛋白伴侣的具体作用仍不清楚。在这里，我们描述了一种新的肌动蛋白结合蛋白，Ciboulot（Cib），在果蝇脑变态的轴突生长中起着重要作用。Cib功能的丧失导致中枢脑中轴突生长缺陷，而在发育过程中该基因的过度表达导致过度生长的投射。Cib蛋白显示出与β-胸腺素很强的序列相似性，但具有类似profilin的生化特性：Cib-肌动蛋白复合物仅在倒刺末端参与肌动蛋白丝组装，Cib增强了体外基于肌动蛋白的运动性。遗传实验表明，Cib和果蝇profilin蛋白Chickadee（Chic）在中枢脑变态中合作。
A dynamic actin cytoskeleton is essential for the remodeling of cell shape during development, but the specific roles of many actin partners remain unclear. Here we characterize a novel actin binding protein, Ciboulot (Cib), which plays a major role in axonal growth during Drosophila brain metamorphosis. Loss of Cib function leads to axonal growth defects in the central brain, while overexpression of the gene during development leads to overgrown projections. The Cib protein displays strong sequence similarity to beta-thymosins but has biochemical properties like profilin: the Cib-actin complex participates in actin filament assembly exclusively at the barbed end, and Cib enhances actin-based motility in vitro. Genetic experiments show that Cib and the Drosophila profilin protein Chickadee (Chic) cooperate in central brain metamorphosis.