Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of Delta
Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of Delta
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DOI：
10.1016/s1534-5807(01)00092-2
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发表时间：
2001-12-01
影响因子：
11.8
通讯作者：
Rubin, GM
中科院分区：
文献类型：
--
作者：
Lai, EC;Deblandre, GA;Rubin, GM
果蝇基因neuralized（neur）长期以来被认为是Notch（N）通路介导的各种过程的正确执行所必需的，但其在该通路中的作用一直是难以捉摸的。在这份报告中，我们提出的遗传和生化证据表明，神经是一个环型，E3泛素连接酶。接下来，我们表明，neur是需要适当的内化DI在发展中的眼睛。最后，我们表明，异位神经细胞的目标DI的内在化和降解在一个环指依赖的方式，这两个存在于一个物理复杂。总的来说，我们的数据表明，神经元是一种泛素连接酶，积极调节N途径，促进内吞和降解的DI。
The Drosophila gene neuralized (neur) has long been recognized to be essential for the proper execution of a wide variety of processes mediated by the Notch (N) pathway, but its role in the pathway has been elusive. In this report, we present genetic and biochemical evidence that Neur is a RING-type, E3 ubiquitin ligase. Next, we show that neur is required for proper internalization of DI in the developing eye. Finally, we demonstrate that ectopic Neur targets DI for internalization and degradation in a RING finger-dependent manner, and that the two exist in a physical complex. Collectively, our data indicate that Neur is a ubiquitin ligase that positively regulates the N pathway by promoting the endocytosis and degradation of DI.