CXCR3‐positive B cells found at elevated frequency in the peripheral blood of patients with MALT lymphoma are attracted by MIG and belong to the lymphoma clone
CXCR3‐positive B cells found at elevated frequency in the peripheral blood of patients with MALT lymphoma are attracted by MIG and belong to the lymphoma clone
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DOI：
10.1002/ijc.20823
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发表时间：
2005-05
影响因子：
6.4
通讯作者：
Hiroaki Suefuji;K. Ohshima;K. Karube;R. Kawano;K. Nabeshima;J. Suzumiya;N. Hayabuchi;M. Kikuchi
中科院分区：
文献类型：
--
作者：
Hiroaki Suefuji;K. Ohshima;K. Karube;R. Kawano;K. Nabeshima;J. Suzumiya;N. Hayabuchi;M. Kikuchi
趋化因子受体通过结合其配体介导淋巴细胞的迁移。CXCR 3在Th 1 T细胞中表达;然而，最近报道CXCR 3在B细胞粘膜相关淋巴组织（MALT）型淋巴瘤和脾边缘区淋巴瘤中表达。为探讨外周血中CXCR 3阳性B淋巴细胞是否迁移至MALT和脾脏，以及淋巴瘤克隆是否存在于PB中，我们研究了16例MALT淋巴瘤。在MALT病例中，PB中的CXCR 3阳性B淋巴细胞可迁移至CXCR 3配体CXCR 3。免疫组化分析显示MALT淋巴瘤细胞表达CXCR 3，而上皮腺体和/或基质细胞表达CXCR 3。在VH基因重排的PCR分析中，MALT淋巴瘤显示单克隆或寡克隆条带。此外，在16例MALT病例中，8例MALT淋巴瘤的VH基因重排与PB中CXCR 3阳性B淋巴细胞具有相同的条带。在4例病例中，在MALT淋巴瘤和PB的CXCR 3阳性B淋巴细胞中确认了相同的DNA序列克隆。研究结果支持MALT患者PB中CXCR 3阳性B淋巴细胞属于淋巴瘤克隆并迁移至表达β-内酰胺酶的粘膜相关淋巴组织的理论。它似乎与MALT淋巴瘤的播散有关。© 2004 Wiley利斯公司
Chemokine receptors mediate the migration of lymphocytes through binding of their ligands. CXCR3 is expressed in Th1 T cells; however, CXCR3 was recently reported in B‐cell mucosa‐associated lymphoid tissue (MALT)‐type lymphoma and splenic marginal zone lymphoma. To investigate whether CXCR3‐positive B lymphocytes in peripheral blood (PB) migrate to MALT and spleen, and whether the lymphoma clone is present in PB, we studied 16 cases of MALT lymphoma. In MALT cases, CXCR3‐positive B lymphocytes in PB could migrate to MIG, the CXCR3 ligand. Immunohistochemical analysis showed that MALT lymphoma cells expressed CXCR3, whereas epithelial glands and/or stromal cells expressed MIG. In the PCR analysis for VH gene rearrangements, MALT lymphoma showed monoclonal or oligoclonal bands. In addition, in 8 of 16 MALT cases, the VH gene rearrangement of MALT lymphoma had the same bands as the CXCR3‐positive B lymphocytes in PB. In 4 cases, the same clones of DNA sequences were confirmed in MALT lymphoma and CXCR3‐positive B lymphocytes of PB. The findings support the theory that CXCR3‐positive B lymphocytes in PB of MALT patients belong to the lymphoma clone and migrate to MIG‐expressing mucosa‐associated lymphoid tissue. It seemed to be associated with the dissemination of MALT lymphoma. © 2004 Wiley‐Liss, Inc.