Bone preparation at the KCCAMS laboratory
Bone preparation at the KCCAMS laboratory
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DOI：
10.1016/j.nimb.2009.10.061
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发表时间：
2010-04-01
影响因子：
1.3
通讯作者：
Taylor, R. E.
中科院分区：
文献类型：
--
作者：
Beaumont, Will;Beverly, Robert;Taylor, R. E.
通过超滤纯化胶原蛋白[1]作为一种从考古骨中去除环境污染物的上级C-14样品制备方法正被广泛接受。我们概述了在KCCAMS实验室（UC Irvine）使用的骨样本制备方法。还讨论了方法开发数据，包括糊化温度试验结果、10 kDa与30 kDa分子量（MW）超滤的评价、超滤器清洁方法的改进、超滤器记忆试验和超滤器甘油污染。这些测试并不是详尽无遗的，而是为了展示如何用相对较少的努力来验证和改进标准程序。(C)2009 Elsevier B. V.保留所有权利。
Collagen purification by ultrafiltration [1] is becoming widely accepted as a superior C-14 sample preparation method for removing environmental contamination from archaeological bone. We present an overview of bone sample preparation methods used at the KCCAMS laboratory, UC Irvine. Methods development data are also discussed, including results of tests of gelatinization temperature, evaluation of 10 kDa vs. 30 kDa molecular weight (MW) ultrafiltration, improvements to ultrafilter cleaning methods, tests of ultrafilter memory, and ultrafilter glycerin contamination. These tests were not intended to be exhaustive, but rather to show how standard procedures can be validated and improved with relatively little effort. (C) 2009 Elsevier B.V. All rights reserved.