Overexpression of Bmi-1 Induces the Malignant Transformation of Gastric Epithelial Cells In Vitro
Overexpression of Bmi-1 Induces the Malignant Transformation of Gastric Epithelial Cells In Vitro
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Bmi-1过表达诱导胃上皮细胞体外恶性转化
DOI：
10.3727/096504013x13786659070316
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Huang, Kaihong
中科院分区：
文献类型：
--
作者：
Chen, Yinting;Lian, Guoda;Huang, Kaihong
癌基因Bmi-1 （b细胞特异性Moloney小鼠白血病病毒整合位点1）因参与多种肿瘤的发生而受到广泛关注。我们前期研究表明，Bmi-1在胃癌中高表达，并与患者预后相关。然而，异常的Bmi-1表达是否对胃上皮细胞的转化至关重要尚不清楚。在这项研究中，我们在人胃上皮永生化细胞系GES-1中稳定表达了Bmi-1。过表达Bmi-1促进细胞生长和增殖，抑制细胞凋亡，增强克隆形成能力，具有不依赖锚定生长的特点，增强迁移和侵袭能力。因此，我们的研究结果表明，Bmi-1的异位表达在胃上皮细胞的恶性转化中发挥了重要作用。
Oncogene Bmi-1 (B-cell-specific Moloney murine leukemia virus integration site 1) has attracted much attention for its involvement in the initiation of a variety of tumors. Our previous study showed that Bmi-1 was highly expressed in gastric cancer and correlated with patient prognosis. However, whether aberrant Bmi-1 expression was critical for the transformation of gastric epithelial cells remains unknown. In this study, we stably expressed Bmi-1 in a human gastric epithelial immortalized cell line, GES-1. The overexpression of Bmi-1 promoted cell growth and proliferation, inhibited apoptosis, enhanced clone formation capability, possessed the characteristics of anchorage-independent growth, and increased migration and invasion abilities. Therefore, our findings demonstrated that ectopic expression of Bmi-1 played an important role in the malignant transformation of gastric epithelial cells.