Immunomodulating activity of exopolysaccharide-producing Leuconostoc mesenteroides strain NTM048 from green peas
Immunomodulating activity of exopolysaccharide-producing Leuconostoc mesenteroides strain NTM048 from green peas
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DOI：
10.1111/jam.12411
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发表时间：
2014-04-01
影响因子：
4
通讯作者：
Hisa, K.
中科院分区：
文献类型：
--
作者：
Matsuzaki, C.;Kamishima, K.;Hisa, K.
目的寻找新型益生菌，增强人体粘膜屏障功能。的有效性进行了评估，在体外和在vivo.Methods和ResultsStimulation的伊加生产在粘膜surfaces. The乳酸菌（LAB）的最有益的性状之一，用于增强屏障。因此，使用鼠派伊尔氏斑细胞评价了173种LAB菌株诱导伊加产生的能力。从绿色豌豆中分离到的菌株NTM 048活性最高，经鉴定为肠膜明串珠菌肠膜明肠系膜发现该菌株耐受胃肠道消化并产生大量具有IgA诱导活性的胞外多糖。膳食补充NTM 048诱导BALB/cA小鼠的粪便伊加含量和血浆伊加水平显著增加。Peyer’s patch细胞的基因表达分析显示，NTM 048摄入上调了转化生长因子-β和激活诱导的胞苷脱氨酶基因。揭示了NTM 048作为益生菌增强粘膜屏障功能的潜力。研究的意义和影响本报告展示了一种食品-适用的肠膜明串珠菌菌株，其分泌表现出高IgA诱导能力的胞外多糖。
AimsThe present work was aimed to find novel probiotics to enhance the mucosal barrier function of humans. The effectiveness was evaluated in vitro and in vivo.Methods and ResultsStimulation of IgA production in mucosal surfaces is one of the most beneficial traits of lactic acid bacteria (LAB) for enhancing the barrier. Therefore, 173 LAB strains were evaluated for the ability to induce IgA production using murine Peyer's patch cells. Strain NTM048 isolated from green peas showed the highest activity and was identified as Leuconostoc mesenteroides subsp. mesenteroides. This strain was found to tolerate gastrointestinal digestion and produce large amounts of exopolysaccharides, which possess IgA-inducing activity. Dietary supplementation with NTM048 induced a significant increase in the faecal IgA content and plasma IgA levels of BALB/cA mice. A gene expression analysis of Peyer's patch cells revealed that the transforming growth factor-beta and activation-induced cytidine deaminase genes were upregulated by NTM048 intake.ConclusionsStrain NTM048 stimulates Peyer's patch cells to induce intestinal and systemic immune response, revealing the potential of NTM048 as a probiotic for enhancing the mucosal barrier function.Significance and Impact of the StudyThis report demonstrates a food-applicable Leuconostoc mesenteroides strain secreting exopolysaccharide that shows high IgA-inducing ability.