Effects of a murine mammary tumor on in vivo and in vitro hemopoiesis.
Effects of a murine mammary tumor on in vivo and in vitro hemopoiesis.
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DOI：
10.1002/stem.5530030203
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
G. Johnson;N. Nicola;R. Whitehead
中科院分区：
文献类型：
--
作者：
G. Johnson;N. Nicola;R. Whitehead
描述了自体移植的乳腺肿瘤（RIII-T3）对RIII小鼠造血的影响。荷瘤动物在接种后30 - 40天死亡，并显示脾肿大、极端嗜中性粒细胞和脾、外周血和骨髓中单核细胞水平中度升高。中性粒细胞和单核细胞的前体细胞，粒细胞/巨噬细胞集落形成细胞（GM-CFC）在脾脏，骨髓和外周血中升高。RIII-T3条件培养基刺激骨髓GM-CFC，并导致骨髓单核细胞系WEHI-3B在体外分化。条件培养基不刺激红细胞、巨核细胞或嗜酸性粒细胞集落形成。当条件培养基进行分级分离时，观察到对应于GM-CSF和G-CSF的两个活性峰，表明在荷瘤动物中观察到的极端嗜中性粒细胞性可能是造血系统长期暴露于这些造血激素所致。
The effects of an autologous transplanted mammary tumor (RIII-T3) on hemopoiesis in RIII mice are described. Tumor-bearing animals died 30 to 40 days after inoculation and displayed splenomegaly, extreme neutrophilia, and moderately increased monocyte levels in the spleen, peripheral blood, and bone marrow. The precursors of neutrophils and monocytes, granulocyte/macrophage colony-forming cells (GM-CFC) were elevated in the spleen, bone marrow, and peripheral blood. RIII-T3-conditioned medium stimulated bone marrow GM-CFC and caused the myelomonocytic cell line, WEHI-3B, to differentiate in vitro. The conditioned medium did not stimulate erythroid, megakaryocyte, or eosinophil colony formation. When conditioned medium was fractionated, two peaks of activity corresponding to GM-CSF and G-CSF were observed, suggesting that the extreme neutrophilia observed in tumor-bearing animals may result from chronic exposure of the hemopoietic system to these hemopoietic hormones.