Postmortem Internal Gas Reservoir Monitoring Using GC×GC-HRTOF-MS
Postmortem Internal Gas Reservoir Monitoring Using GC×GC-HRTOF-MS
复制标题
使用 GC×GC-HRTOF-MS 进行死后内部气藏监测
DOI：
10.3390/separations3030024
复制
发表时间：
2016
期刊：
影响因子：
2.6
通讯作者：
J. Focant
中科院分区：
文献类型：
--
作者：
Pierre‐Hugues Stefanuto;Katelynn A. Perrault;S. Grabherr;V. Varlet;J. Focant
法医调查往往需要对尸体进行尸检。然而，尸检过程中的证据收集往往是破坏性的，这意味着尸体无法再以原始状态进行检查。为了获得身体的内部图像，全身死后计算机断层扫描（PMCT）已被证明是一种有价值的非破坏性工具，目前在法医学中心使用。PMCT还可以用于视觉定位尸体内的气体储存器，通过分析可以提供有关死后产生的非常挥发性化合物的有用信息。然而，从未尝试过对这些储层中存在的所有潜在挥发性有机化合物（VOC）进行非靶向分析。本研究的目的是调查这些水库的挥发性有机化合物概况，并评估这些信息的潜在用途，以记录周围的死亡，死亡原因和尸体埋藏的情况。采用全二维气相色谱-飞行时间高分辨质谱联用技术（GC×GC-HRTOF-MS）测定挥发性有机化合物。这项研究表明，气体储层中VOC的化学成分在单个身体内的不同位置之间以及个体之间存在差异。在未来，这项工作可以扩大到研究一种新的，非破坏性的尸体筛选方法之前，全面的尸检程序。
Forensic investigations often require postmortem examination of a body. However, the collection of evidence during autopsy is often destructive, meaning that the body can no longer be examined in its original state. In order to obtain an internal image of the body, whole body postmortem computed tomography (PMCT) has proven to be a valuable non-destructive tool and is currently used in medicolegal centers. PMCT can also be used to visually locate gas reservoirs inside a cadaver, which upon analysis can provide useful information regarding very volatile compounds that are produced after death. However, the non-targeted profiling of all potential volatile organic compounds (VOCs) present in these reservoirs has never been attempted. The aim of this study was to investigate the VOC profile of these reservoirs and to evaluate potential uses of such information to document circumstances surrounding death, cause of death and body taphonomy. Comprehensive two-dimensional gas chromatography coupled to time-of-flight high-resolution mass spectrometry (GC×GC-HRTOF-MS) was used for VOC measurements. This study demonstrated that the chemical composition of VOCs within the gas reservoirs differed between locations within a single body but also between individuals. In the future, this work could be expanded to investigate a novel, non-destructive cadaver screening approach prior to full autopsy procedures.