Immobilization and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods
Immobilization and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods
复制标题
旋转组织培养法快速形成肝细胞球体的固定化和长期白蛋白分泌
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
Motoyuki Suzuki
中科院分区：
文献类型：
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作者：
Y. Sakai;K. Furukawa;Motoyuki Suzuki
通过旋转组织培养方法形成的原代培养物中成年大鼠肝细胞的球状聚集体（球状体）。确定了终止轮作补播的适宜时间。所形成的球状体被稳定地固定到聚赖氨酸包被的表面上，并且与通过静止培养形成的球状体相比，它们以几乎相同的水平分泌白蛋白约2周。
Spheroidal aggregates (spheroids) of adult rat hepatocytes in primary culture formed by rotational tissue culture methods. The time suitable for terminating the rotation and reseeding was determined. The spheroids formed were immobilized stably onto polylysine-coated surfaces, and they secreted albumin for about 2 weeks at almost the same level as compared to spheroids formed by stationary culture.