Infrared laser ablation sampling coupled with data independent high resolution UPLC-IM-MS/MS for tissue analysis
Infrared laser ablation sampling coupled with data independent high resolution UPLC-IM-MS/MS for tissue analysis
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DOI：
10.1016/j.aca.2018.06.066
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发表时间：
2018-11-30
影响因子：
6.2
通讯作者：
Solouki, Touradj
中科院分区：
文献类型：
--
作者：
Pettit, Michael E.;Donnarumma, Fabrizio;Solouki, Touradj
采用红外激光消融微采样技术，结合数据依赖采集（DDA）和离子迁移增强数据独立采集（HDMSE），对大鼠脑组织进行质谱自下而上的蛋白质组学分析。对12个激光消融组织切片的HDMSE和DDA分析结果显示，HDMSE鉴定出的多肽和蛋白质的数量是DDA的7倍。为了评估超高效液相色谱（UPLC）峰阻塞对HDMSE和DDA分析的影响，在6个不同的UPLC分离时间对大鼠脑全组织消化液进行了分析。与激光烧蚀样品的结果类似，在所有六次UPLC分离时间内，全组织消化的HDMSE分析产生的蛋白质鉴定量约为DDA的四倍。(C) 2018 Elsevier B.V.版权所有
Infrared laser ablation microsampling was used with data-dependent acquisition (DDA) and ion mobility-enhanced data-independent acquisition (HDMSE) for mass spectrometry based bottom-up proteomics analysis of rat brain tissue. Results from HDMSE and DDA analyses of the 12 laser ablation sampled tissue sections showed that HDMSE consistently identified approximately seven times more peptides and four times more proteins than DDA. To evaluate the impact of ultra-performance liquid chromatography (UPLC) peak congestion on HDMSE and DDA analysis, whole tissue digests from rat brain were analyzed at six different UPLC separation times. Analogous to results from laser ablated samples, HDMSE analyses of whole tissue digests yielded about four times more proteins identified than DDA for all six UPLC separation times. (C) 2018 Elsevier B.V. All rights reserved.