Identification of target genes for a prolactin family paralog in mouse decidua.
Identification of target genes for a prolactin family paralog in mouse decidua.
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DOI：
10.1530/rep-15-0107
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发表时间：
2015-06
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影响因子：
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通讯作者：
Soares MJ
中科院分区：
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作者：
Alam SM;Konno T;Soares MJ
催乳素家族8，亚家族a，成员2（PRL 8A 2;也称为蜕膜催乳素相关蛋白; dPRP）是扩展催乳素家族的成员。PRL 8A 2在子宫蜕膜中表达，并有助于对缺氧的妊娠依赖性适应。本研究的目的是确定子宫胎盘室内PRL 8A 2作用的基因靶点。对来自野生型和Prl 8a 2缺失组织的RNA样品进行AffyphidDNA微阵列分析。使用定量RT-PCR进行DNA微阵列的验证。9个基因在Prl 8a 2无效组织中被证实具有降低的表达（例如Klk 7、Rimklb、Arhgef 6、Calm 4、Sprr 2 h、Prl 4al、Ccl 27、Lipg和Htra 3）。这些包括潜在的蜕膜，内皮细胞和滋养层细胞的目标正调节PRL 8A 2。在Prl 8a 2无效组织中鉴定了Derl 3、Herpud 1、Creld 2、Hsp 90 b1、Ddit 3和Hspa 5的显著上调，反映了增加的内质网（ER）应激反应。ER应激基因在子宫蜕膜中表达显著。我们建议，PRL 8A 2是一个介导的孕甾酮依赖性调节子宫内的生理应激反应。
Prolactin family 8, subfamily a, member 2 (PRL8A2; also called decidual prolactin-related protein; dPRP) is a member of the expanded prolactin family. PRL8A2 is expressed in the uterine decidua and contributes to pregnancy-dependent adaptations to hypoxia. The purpose of this study was to identify gene targets for PRL8A2 action within the uteroplacental compartment. Affymetrix DNA microarray analysis was performed for RNA samples from wild type and Prl8a2 null tissues. Validation of the DNA microarray was performed using quantitative RT-PCR. Nine genes were confirmed with decreased expression in Prl8a2 null tissues (e.g. Klk7, Rimklb, Arhgef6, Calm4, Sprr2h, Prl4a1, Ccl27, Lipg, and Htra3). These include potential decidual, endothelial, and trophoblast cell targets positively regulated by PRL8A2. A significant upregulation of Derl3, Herpud1, Creld2, Hsp90b1, Ddit3, and Hspa5 was identified in Prl8a2 null tissues, reflecting an increased endoplasmic reticulum (ER) stress response. ER stress genes were prominently expressed in the uterine decidua. We propose that PRL8A2 is a mediator of progesterone-dependent modulation of intrauterine responses to physiological stressors.