Paradoxical increase in the bispectral index during deep anesthesia in New Zealand white rabbits.
Paradoxical increase in the bispectral index during deep anesthesia in New Zealand white rabbits.
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发表时间：
2014
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通讯作者：
A. Romanov;Rajinder-Singh Moon;Mei Wang;S. Joshi
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作者：
A. Romanov;Rajinder-Singh Moon;Mei Wang;S. Joshi
麻醉水平的客观监测在仔细控制的转化神经科学研究中至关重要。脑电双频指数（BIS）在监测人体麻醉中的有用性已得到充分证实。然而，其应用的有效性尚未在实验室动物中得到证实。我们在8只新西兰白色家兔上评估了BIS是否可以可靠地用于监测深度麻醉。实验基线麻醉维持持续输注丙泊酚和异氟烷，这两种药物都滴定到EEG活动。将家兔随机分配接受3种浓度递增的常用麻醉药物（依托咪酯、丙泊酚和异氟烷），目的是产生EEG活动的爆发抑制，产生至少10 s的持续EEG沉默。家兔在激发之间有20分钟的恢复间隔。在每只动物中，诱导短暂脑灌注不足以产生由于缺血引起的可逆EEG沉默作为第四次激发，随后是终末停搏。分析每只家兔的BIS、EEG和生理数据。我们观察到稳定的BIS值在40至60的范围内，在管理的基线麻醉。然而，随着麻醉深度的加深，麻醉药物的挑战，BIS值矛盾地随着剂量的增加而增加。BIS信号质量指数下降，而总功率下降。与这些意想不到的结果相反，BIS值在终末停搏期间迅速下降至接近0，如预期的那样。因此，我们不认为BIS是监测实验室家兔深麻醉水平的有用方法。
Objective monitoring of the level of anesthesia is crucial in carefully controlled translational neuroscience studies. The usefulness of bispectral index (BIS) in monitoring human anesthesia is well established. However, the validity of its application remains unproven in laboratory animals. We assessed whether BIS could be used reliably in monitoring the depth of deep anesthesia in 8 New Zealand white rabbits. Experimental baseline anesthesia was maintained with continuous infusion of propofol and administration of isoflurane, both of which were titrated to EEG activity. The rabbits were allocated randomly to receive 3 increasing concentrations of common anesthetic drugs (etomidate, propofol, and isoflurane) aimed to produce burst suppression of EEG activity yielding at least 10 s of sustained EEG silence. Rabbits had a 20-min recovery interval between challenges. Transient cerebral hypoperfusion to produce reversible EEG silence due to ischemia was induced as a fourth challenge, followed by terminal arrest, in each animal. BIS, EEG, and physiologic data were analyzed for each rabbit. We observed stable BIS values in the range of 40 to 60 during the administration of baseline anesthesia. However, as the depth of anesthesia deepened with the anesthetic drug challenges, the BIS value paradoxically increased with increasing doses. The BIS signal quality index declined while the total power decreased. In contrast to these unexpected results, BIS values decreased rapidly to near 0 during terminal arrest, as expected. Therefore, we do not consider BIS to be a useful method for monitoring deep levels of anesthesia in laboratory rabbits.