CORNEAL DONOR INFECTION BY HERPES-SIMPLEX VIRUS - HERPES-SIMPLEX VIRUS-DNA IN DONOR CORNEAS
CORNEAL DONOR INFECTION BY HERPES-SIMPLEX VIRUS - HERPES-SIMPLEX VIRUS-DNA IN DONOR CORNEAS
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DOI：
10.1097/00003226-199407000-00003
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发表时间：
1994-07-01
期刊：
影响因子：
2.8
通讯作者：
TULLO, AB
中科院分区：
文献类型：
--
作者：
CLEATOR, GM;KLAPPER, PE;TULLO, AB
三个角膜椎间盘（来自两个供体）在常规的“长期”器官培养储存过程中发生了几乎全部的内皮细胞丢失。这些角膜的实验室研究显示有单纯疱疹病毒（HSV）感染的证据。其中一名捐赠者的另一块角膜已被发给一名圆锥角膜患者进行移植。术后5天发现移植物恶化；椎间盘在2个月时被切除，显示感染了HSV。在一项旨在模拟供体球体初始“清洗”的实验中，0.1%的聚乙烯吡咯烷酮碘保护细胞免受HSV感染。结论：在这些角膜中检测到HSV不能用眼表面的外部污染来解释。此外，从47个连续献血者身上采集的结膜和咽拭子的培养证实，HSV很少在死亡时或死亡前后被分离出来。随机选择5对用于移植的供体角膜，并调查HSV的存在。用聚合酶链反应（PCR）在两名角膜供体组织中检测HSV DNA。顺序逐级切片提示，存在时HSV DNA分布在角膜内的离散灶中。这些观察结果表明，HSV感染可能是角膜器官培养过程中严重内皮细胞丢失的原因，并可能为角膜移植的一些“失败”提供解释。
Three corneoscleral discs (from two donors) underwent subtotal endothelial loss during routine ''long-term'' organ culture storage. Laboratory studies of these corneas revealed evidence of herpes simplex virus (HSV) infection. The fellow cornea from one of the donors had been issued for transplant to a patient with keratoconus. Deterioration of the graft was noted 5 days after surgery; the disc was removed at 2 months and was shown to be infected with HSV. In an experiment designed to simulate initial ''cleansing'' of donor globes, 0.1% polyvinylpyrolidone-iodine protected cells from infection with HSV. It was concluded that the detection of HSV in these corneas could not be explained by external contamination of the ocular surface. Furthermore, culture of conjunctival and pharangeal swabs taken from 47 consecutive donors confirmed that HSV is rarely isolated at or around the time of death. Five pairs of donor corneas destined for use in transplantation were selected at random and investigated for the presence of HSV. HSV DNA was detected by polymerase chain reaction (PCR) in tissue from two of the corneal donors. Sequential stepwise sectioning suggested that HSV DNA when present was distributed in discrete foci within the cornea. These observations suggest that HSV infection may be a cause of severe endothelial loss during corneal organ culture and possibly provide an explanation for some ''failures'' of corneal grafting.