Cyborg lectins: novel leguminous lectins with unique specificities.
Cyborg lectins: novel leguminous lectins with unique specificities.
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Cyborg 凝集素：具有独特特异性的新型豆科植物凝集素。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
T. Osawa
中科院分区：
文献类型：
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作者：
K. Yamamoto;I. Maruyama;T. Osawa
紫荆花凝集素(BPA)是一种与β-半乳糖结合的豆科凝集素。豆科凝集素含有一个长的金属结合环，其中一部分决定了它们与碳水化合物结合的特异性。随机突变被引入到编码BPA的一部分cDNA中，该部分对应于凝集素的碳水化合物结合环。用淘选法筛选了Lambda foo噬菌体表面表达的突变型凝集素文库。分离到几个对甘露糖或N-乙酰氨基葡萄糖有亲和力的噬菌体克隆。这些结果表明，制造具有独特和所需的碳水化合物结合特性的人工凝集素(所谓的“半人凝集素”)是可能的。
Bauhinia purpurea lectin (BPA) is one of the beta-galactose-binding leguminous lectins. Leguminous lectins contain a long metal-binding loop, part of which determines their carbohydrate-binding specificities. Random mutations were introduced into a portion of the cDNA coding BPA that corresponds to the carbohydrate-binding loop of the lectin. An library of the mutant lectin expressed on the surface of lambda foo phages was screened by the panning method. Several phage clones with an affinity for mannose or N-acetylglucosamine were isolated. These results indicate the possibility of making artificial lectins (so-called "cyborg lectins") with distinct and desired carbohydrate-binding specificities.