Involvement of Epidermis Cell Proliferation in Defense Against Beauveria bassiana Infection.
Involvement of Epidermis Cell Proliferation in Defense Against Beauveria bassiana Infection.
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表皮细胞增殖参与防御白僵菌感染
DOI：
10.3389/fimmu.2021.741797
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发表时间：
2021
影响因子：
7.3
通讯作者：
Ling E
中科院分区：
文献类型：
--
作者：
Huang W;Tang R;Li S;Zhang Y;Chen R;Gong L;Wei X;Tang Y;Liu Q;Geng L;Pan G;Beerntsen BT;Ling E
球孢白僵菌是一种能侵染多种昆虫的病原真菌，在世界范围内被广泛用作生物农药。在到达血腔之前，B。球孢必须穿透由厚几丁质层和表皮细胞组成的珠被。B分泌几丁质酶、蛋白酶和脂肪酶。球孢可能参与真菌侵入珠被。虽然需要显微镜证据，但难以按照传统的浸泡感染方法定位精确的感染部位。因此，我们开发了一种新的方法，将分生孢子溶液固定在一个片段的背面。这种固定部位感染方法具有致病性，但也具有剂量依赖性。使用固定部位感染方案，通过光学和透射电子显微镜跟踪角质层内的菌丝也非常方便。用真菌萌发过程中分泌的蛋白酶ΔBPS8固定位点感染几丁质层后，在几丁质层内检测到很少的菌丝，这一事实表明该方法适用于大规模筛选参与穿透珠被的基因。我们还发现，黑化发生在新菌丝穿透几丁质层之前。最重要的是，我们发现真菌感染可以通过DNA复制和细胞分裂诱导表皮细胞增殖，这是宿主抵御真菌感染所必需的。因此，定位侵染法有助于明确真菌与寄主在珠被中的相互作用机制，从而更有效地发挥真菌的生物毒力。
Entomopathogenic fungi Beauveria bassiana can infect many species of insects and is used as a biological pesticide world-wide. Before reaching the hemocoel, B. bassiana has to penetrate the integument which is composed of a thick chitin layer and epidermal cells. Some chitinase, protease and lipase secreted by B. bassiana are probably involved in the fungal penetration of the integument. While microscopic proof is needed, it is difficult to locate the precise infection sites following the traditional method of immersion infection. Consequently, we developed a new method to inoculate conidia solution into a single fixed-site on the back of one segment. This fixed-site infection method is pathogenic but it is also dose dependent. Using the fixed-site infection protocol, it is also very convenient to track hyphae inside the cuticle layer by light and transmission electron microscopy. The fact that few hyphae were detected inside the chitin layer after fixed-site infection with mutant ΔBPS8, a protease secreted during fungi germination, indicates that this method is suitable for screening genes involved in penetrating the integument in large scale. We also found that melanization occurs before new hyphae penetrate the chitin layer. Most importantly, we discovered that fungal infection can induce epidermal cell proliferation through DNA duplication and cell division, which is essential for the host to defend against fungal infection. Taken together the fixed-site infection method may be helpful to determine the mechanism of fungal and host interaction in the integument so as to effectively exert fungal biological virulence.
影响因子：
5.7
作者：
Wang ZL;Ying SH;Feng MG
通讯作者：
Feng MG