Hymenoptera flight muscle mitochondrial function: Increasing metabolic power increases oxidative stress
Hymenoptera flight muscle mitochondrial function: Increasing metabolic power increases oxidative stress
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DOI：
10.1016/j.cbpa.2019.01.002
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发表时间：
2019-04-01
影响因子：
2.3
通讯作者：
Hickey, Anthony J. R.
中科院分区：
文献类型：
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作者：
Hedges, Christopher P.;Wilkinson, Reuben T.;Hickey, Anthony J. R.
昆虫飞行是一项高强度的活动，但生物力学和代谢要求可能会因生活方式和摄食模式而异。例如，蜜蜂通常以花粉和花蜜为食，而黄蜂也积极狩猎和捕食重猎物。代谢需求的这些变化可能导致飞行肌肉中代谢途径的不同能力，并且利用这些途径中的一些可能以增加自由基产生为代价。为了研究代谢要求和氧化应激如何在物种之间变化，我们探讨了蜜蜂（意大利蜜蜂），熊蜂（熊蜂terrestris）和德国黄蜂（Vespula germanica）的飞行力学和代谢的变化。机翼结构和飞行肌肉的性能进行了比较旁边的措施的氧通量和活性氧（ROS）的生产透化的飞行肌肉。黄蜂的机翼结构最适合承载重载荷，具有最高的机翼展弦比，最低的机翼载荷和最高的飞行肌肉比。大黄蜂的翼展弦比和飞行肌肉比最低，翼载荷最高。虽然黄蜂在氧化磷酸化过程中也有最高的耗氧率，但化学解偶联后黄蜂肌肉的耗氧率并没有增加，而两种蜜蜂的耗氧率却增加了。虽然线粒体甘油3-磷酸脱氢酶（mGPDH）介导的氧通量是最大的黄蜂，肌纤维释放大量的活性氧通过这一途径。总的来说，黄蜂通过改变翅膀和飞行肌肉质量以及最大化OXPHOS能力来最大化提升能力，这伴随着ROS的产生。
Insect flight is a high intensity activity, but biomechanical and metabolic requirements may vary depending on life style and feeding mode. For example, bees generally feed on pollen and nectar, whereas wasps also actively hunt and scavenge heavy prey. These variations in metabolic demands may result in different capacities of metabolic pathways in flight muscle, and utilisation some of these pathways may come at a cost of increased free radical production. To examine how metabolic requirements and oxidative stress vary between species, we explored the variation in flight mechanics and metabolism of the honeybee (Apis mellifera), bumblebee (Bombus terrestris), and German wasp (Vespula germanica). Wing structures and flight muscle properties were compared alongside measures of oxygen flux and reactive oxygen species (ROS) production from permeabilised flight muscle. The wasp wing structure is best adapted for carrying heavy loads, with the highest wing aspect ratio, lowest wing loading, and highest flight muscle ratio. Bumblebees had the lowest wing aspect ratio and flight muscle ratio, and highest wing loading. Although wasps also had the highest rates of oxygen consumption during oxidative phosphorylation, oxygen consumption did not increase in the wasp muscle following chemical uncoupling, while it did for the two bee species. While mitochondrial glycerol 3-phosphate dehydrogenase (mGPDH) mediated oxygen flux was greatest in wasps, muscle fibres released greater amounts of ROS through this pathway. Overall, the wasp has maximised lifting capacities through varying wing and flight muscle mass and by maximising OXPHOS capacities, and this accompanies elevated ROS production.