ANTIBODY-RESPONSES OF GUINEA-PIGS, RABBITS AND PIGS TO INACTIVATED PORCINE PARVOVIRUS VACCINES
ANTIBODY-RESPONSES OF GUINEA-PIGS, RABBITS AND PIGS TO INACTIVATED PORCINE PARVOVIRUS VACCINES
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DOI：
10.1016/0378-1135(84)90076-2
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发表时间：
1984-01-01
影响因子：
3.3
通讯作者：
LEMAN, AD
中科院分区：
文献类型：
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作者：
JOO, HS;MOLITOR, TW;LEMAN, AD
比较了豚鼠、兔和猪接种灭活猪细小病毒（PPV）疫苗后的抗体反应。豚鼠、家兔和猪第1次接种后1周的平均PPV血凝抑制（HI）抗体滴度分别为52、56和36，第2次接种后2周的平均抗体滴度分别为896、640和512。根据血凝（HA）单位测定，用更高浓度的病毒制备的PPV疫苗和AlOH凝胶佐剂可在豚鼠中诱导更高的HI抗体滴度。诱导持续高抗体滴度的最佳浓度为HA滴度为256/0.1 ml的疫苗病毒和终浓度为50%的凝胶佐剂。与第一次接种疫苗后2或3周相比，第4周接种第二次疫苗可导致更高的平均HI滴度。这些数据提供了关于使用豚鼠或兔子作为实验动物模型来测试PPV疫苗效力的初步信息。
Antibody responses were compared in guinea pigs, rabbits and pigs following vaccination with inactivated porcine parvovirus (PPV) vaccines. Mean PPV hemagglutination inhibition (HI) antibody titers of 52, 56 and 36 at 1 wk after 1st vaccination, and 896, 640 and 512 at 2 wk after 2nd vaccination were detected in guinea pigs, rabbits, and pigs, respectively. PPV vaccines prepared with greater concentrations of virus, as determined by hemagglutination (HA) units, and with AlOH gel adjuvant, induced higher HI antibody titers in guinea pigs. Optimal concentrations for inducing consistently high antibody titers consisted of vaccine virus with a HA titer of 256/0.1 ml and gel adjuvant at a final concentration of 50%. A 2nd vaccination at 4 wk compared to 2 or 3 wk after 1st vaccination resulted in higher mean HI titers. These data provide preliminary information on the use of guinea pigs or rabbits as laboratory animal models for testing the potency of PPV vaccines.